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Literature summary for 3.1.1.75 extracted from

  • Saegusa, H.; Shiraki, M.; Kanai, C.; Saito, T.
    Cloning of an intracellular poly[D(-)-3-hydroxybutyrate] depolymerase gene from Ralstonia eutropha H16 and characterization of the gene product (2001), J. Bacteriol., 183, 94-100.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Cupriavidus necator 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator no hydrolysis of crystalline poly(3-hydroxybutyrate) poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?
poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 no hydrolysis of crystalline poly(3-hydroxybutyrate) poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Cupriavidus necator
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O no hydrolysis of crystalline poly(3-hydroxybutyrate) Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?
poly[(R)-3-hydroxybutanoate]n + H2O no hydrolysis of crystalline poly(3-hydroxybutyrate) Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?