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Literature summary for 3.1.1.75 extracted from

  • Kasuya, K.; Ohura, T.; Masuda, K.; Doi, Y.
    Substrate and binding specificities of bacterial polyhydroxybutyrate depolymerases (1999), Int. J. Biol. Macromol., 24, 329-336.
    View publication on PubMed
Search for more literature for 3.1.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alcaligenes faecalis
expression in Escherichia coli Pseudomonas stutzeri
expression in Escherichia coli Delftia acidovorans

Protein Variants

Protein Variants Comment Organism
additional information construction of GST-catalytic domain fusionproteins, strong adsorption to polyester granules of poly(3-hydroxybutyrate), poly(3-hydroxypropionate) and poly(2-hydroxypropionate) Alcaligenes faecalis
additional information construction of GST-catalytic domain fusionproteins, strong adsorption to polyester granules of poly(3-hydroxybutyrate), poly(3-hydroxypropionate) and poly(2-hydroxypropionate) Pseudomonas stutzeri
additional information construction of GST-catalytic domain fusionproteins, strong adsorption to polyester granules of poly(3-hydroxybutyrate), poly(3-hydroxypropionate) and poly(2-hydroxypropionate) Delftia acidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Alcaligenes faecalis
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Pseudomonas stutzeri
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Delftia acidovorans
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Delftia acidovorans
-
-
-
Pseudomonas stutzeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Alcaligenes faecalis
recombinant protein Pseudomonas stutzeri
recombinant protein Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxypropionate)n + H2O
-
 Alcaligenes faecalis poly(3-hydroxypropionate)n-x + poly(3-propionate)
-
 ?
poly(3-hydroxypropionate)n + H2O
-
 Pseudomonas stutzeri poly(3-hydroxypropionate)n-x + poly(3-propionate)
-
 ?
poly(3-hydroxypropionate)n + H2O
-
 Delftia acidovorans poly(3-hydroxypropionate)n-x + poly(3-propionate)
-
 ?
poly(4-hydroxybutyrate)n + H2O
-
 Alcaligenes faecalis poly(4-hydroxybutyrate)n-x + poly(4-hydroxybutyrate)
-
 ?
poly(4-hydroxybutyrate)n + H2O
-
 Pseudomonas stutzeri poly(4-hydroxybutyrate)n-x + poly(4-hydroxybutyrate)
-
 ?
poly(4-hydroxybutyrate)n + H2O
-
 Delftia acidovorans poly(4-hydroxybutyrate)n-x + poly(4-hydroxybutyrate)
-
 ?
poly(ethylene adipate)n + H2O
-
 Alcaligenes faecalis poly(ethylene adipate)n-x + poly(ethylene adipate)
-
 ?
poly(ethylene adipate)n + H2O
-
 Pseudomonas stutzeri poly(ethylene adipate)n-x + poly(ethylene adipate)
-
 ?
poly(ethylene adipate)n + H2O
-
 Delftia acidovorans poly(ethylene adipate)n-x + poly(ethylene adipate)
-
 ?
poly(ethylene succinate)n + H2O
-
 Alcaligenes faecalis poly(ethylene succinate)n-x + poly(ethylene succinate)
-
 ?
poly(ethylene succinate)n + H2O
-
 Pseudomonas stutzeri poly(ethylene succinate)n-x + poly(ethylene succinate)
-
 ?
poly(ethylene succinate)n + H2O
-
 Delftia acidovorans poly(ethylene succinate)n-x + poly(ethylene succinate)
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Alcaligenes faecalis poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Pseudomonas stutzeri poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Delftia acidovorans poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?