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Literature summary for 3.1.1.75 extracted from

  • Ohura, T.; Kasuya, K.I.; Doi, Y.
    Cloning and characterization of the polyhydroxybutyrate depolymerase gene of Pseudomonas stutzeri and analysis of the function of substrate-binding domains (1999), Appl. Environ. Microbiol., 65, 189-197.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.75

Cloned(Commentary)

Cloned (Comment) Organism
phaZ PSTgene product, expression in Escherichia coli Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
additional information construction of several GST-fusion domains, analysis of substrate-binding domains Pseudomonas stutzeri

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas stutzeri
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Pseudomonas stutzeri
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Pseudomonas stutzeri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid
-
 ?
methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid
-
 ?
methyl (3R)-3-[[(3'R)-3'-methoxybutanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-methoxybutanoic acid + methyl-(R)-3-hydroxybutanoate + methyl-[[(R)-3-methoxybutanoyl]oxy]butanoate
-
 ?
methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + (R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoic acid
-
 ?
methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-[[(3'''R)-3'''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + (R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-[[(3'''R)-3'''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoyl]oxy]butanoate
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Pseudomonas stutzeri poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?