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Literature summary for 3.1.1.74 extracted from

  • Ahmed Al-Tammar, K.; Omar, O.; Abdul Murad, A.; Abu Bakar, F.
    Expression and characterization of a cutinase (AnCUT2) from Aspergillus niger (2016), Open Life Sci., 11, 29-38 .
No PubMed abstract available
Search for more literature for 3.1.1.74

Application

Application Comment Organism
degradation enzyme is able to modify the surface of the polycaprolactone and polyethylene terephthalate synthetic polyesters Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: dithiothreitol, EDTA, urea Aspergillus niger
PMSF 1 mM, less than 20% residual activity Aspergillus niger
SDS 1 mM, less than 5% residual activity Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A0A0F6TMI2 isoform Cut2
-
Aspergillus niger ATCC 10574 A0A0F6TMI2 isoform Cut2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butanoate + H2O
-
 Aspergillus niger 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl butanoate + H2O
-
 Aspergillus niger ATCC 10574 4-nitrophenol + butanoate
-
 ?
poly(caprolactone) + H2O
-
 Aspergillus niger ?
-
 ?
poly(caprolactone) + H2O
-
 Aspergillus niger ATCC 10574 ?
-
 ?
polyethylene terephthalate + H2O
-
 Aspergillus niger ?
-
 ?
polyethylene terephthalate + H2O
-
 Aspergillus niger ATCC 10574 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 2767, calculated from sequence, x * 40000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, more than 80% residual activity Aspergillus niger
50
-
 1 h, 40% residual activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10 1 h, more than 60% residual activity Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger calculated from sequence
-
 4.7