Application | Comment | Organism |
---|---|---|
synthesis | the cutinase exhibits strong synthetic activity for butyl butanoate in non-aqueous environment,under the optimized reaction conditions esterification efficiency of 95% is observed | Thermothielavioides terrestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
4-nitrophenyl caproate | pH 4.0, 50°C | Thermothielavioides terrestris | |
1.1 | - |
4-nitrophenyl butanoate | pH 4.0, 50°C | Thermothielavioides terrestris | |
8.3 | - |
4-nitrophenyl acetate | pH 4.0, 50°C | Thermothielavioides terrestris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27900 | - |
gel filtration | Thermothielavioides terrestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothielavioides terrestris | - |
- |
- |
Thermothielavioides terrestris CAU709 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermothielavioides terrestris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
983 | - |
substrate 4-nitrophenyl butanoate, pH 4.0, 50°C | Thermothielavioides terrestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Thermothielavioides terrestris | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Thermothielavioides terrestris CAU709 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Thermothielavioides terrestris | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Thermothielavioides terrestris CAU709 | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Thermothielavioides terrestris | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Thermothielavioides terrestris CAU709 | 4-nitrophenol + caproate | - |
? | |
butanol + butanoate | - |
Thermothielavioides terrestris | butyl butanoate + H2O | - |
? | |
tributyrin + H2O | - |
Thermothielavioides terrestris | ? | - |
? | |
tributyrin + H2O | - |
Thermothielavioides terrestris CAU709 | ? | - |
? | |
tricaproin + H2O | - |
Thermothielavioides terrestris | ? | - |
? | |
tricaproin + H2O | - |
Thermothielavioides terrestris CAU709 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27300, SDS-PAGE | Thermothielavioides terrestris |
Synonyms | Comment | Organism |
---|---|---|
CutB | - |
Thermothielavioides terrestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Thermothielavioides terrestris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
more than 30% residual activity | Thermothielavioides terrestris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to | Thermothielavioides terrestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
4-nitrophenyl acetate | pH 4.0, 50°C | Thermothielavioides terrestris | |
0.61 | - |
4-nitrophenyl caproate | pH 4.0, 50°C | Thermothielavioides terrestris | |
0.85 | - |
4-nitrophenyl butanoate | pH 4.0, 50°C | Thermothielavioides terrestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Thermothielavioides terrestris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 10.5 | - |
Thermothielavioides terrestris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
4-nitrophenyl acetate | pH 4.0, 50°C | Thermothielavioides terrestris | |
0.68 | - |
4-nitrophenyl caproate | pH 4.0, 50°C | Thermothielavioides terrestris | |
0.77 | - |
4-nitrophenyl butanoate | pH 4.0, 50°C | Thermothielavioides terrestris |