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Literature summary for 3.1.1.74 extracted from

  • Xu, H.; Yan, Q.; Duan, X.; Yang, S.; Jiang, Z.
    Characterization of an acidic cold-adapted cutinase from Thielavia terrestris and its application in flavor ester synthesis (2015), Food Chem., 188, 439-445 .
    View publication on PubMed

Application

Application Comment Organism
synthesis the cutinase exhibits strong synthetic activity for butyl butanoate in non-aqueous environment,under the optimized reaction conditions esterification efficiency of 95% is observed Thermothielavioides terrestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
4-nitrophenyl caproate pH 4.0, 50°C Thermothielavioides terrestris
1.1
-
4-nitrophenyl butanoate pH 4.0, 50°C Thermothielavioides terrestris
8.3
-
4-nitrophenyl acetate pH 4.0, 50°C Thermothielavioides terrestris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27900
-
gel filtration Thermothielavioides terrestris

Organism

Organism UniProt Comment Textmining
Thermothielavioides terrestris
-
-
-
Thermothielavioides terrestris CAU709
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermothielavioides terrestris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
983
-
substrate 4-nitrophenyl butanoate, pH 4.0, 50°C Thermothielavioides terrestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Thermothielavioides terrestris 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Thermothielavioides terrestris CAU709 4-nitrophenol + acetate
-
?
4-nitrophenyl butanoate + H2O
-
Thermothielavioides terrestris 4-nitrophenol + butanoate
-
?
4-nitrophenyl butanoate + H2O
-
Thermothielavioides terrestris CAU709 4-nitrophenol + butanoate
-
?
4-nitrophenyl caproate + H2O
-
Thermothielavioides terrestris 4-nitrophenol + caproate
-
?
4-nitrophenyl caproate + H2O
-
Thermothielavioides terrestris CAU709 4-nitrophenol + caproate
-
?
butanol + butanoate
-
Thermothielavioides terrestris butyl butanoate + H2O
-
?
tributyrin + H2O
-
Thermothielavioides terrestris ?
-
?
tributyrin + H2O
-
Thermothielavioides terrestris CAU709 ?
-
?
tricaproin + H2O
-
Thermothielavioides terrestris ?
-
?
tricaproin + H2O
-
Thermothielavioides terrestris CAU709 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27300, SDS-PAGE Thermothielavioides terrestris

Synonyms

Synonyms Comment Organism
CutB
-
Thermothielavioides terrestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Thermothielavioides terrestris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
-
more than 30% residual activity Thermothielavioides terrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
stable up to Thermothielavioides terrestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.58
-
4-nitrophenyl acetate pH 4.0, 50°C Thermothielavioides terrestris
0.61
-
4-nitrophenyl caproate pH 4.0, 50°C Thermothielavioides terrestris
0.85
-
4-nitrophenyl butanoate pH 4.0, 50°C Thermothielavioides terrestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Thermothielavioides terrestris

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 10.5
-
Thermothielavioides terrestris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
4-nitrophenyl acetate pH 4.0, 50°C Thermothielavioides terrestris
0.68
-
4-nitrophenyl caproate pH 4.0, 50°C Thermothielavioides terrestris
0.77
-
4-nitrophenyl butanoate pH 4.0, 50°C Thermothielavioides terrestris