Any feedback?
Literature summary for 3.1.1.74 extracted from
- Kinetic cutinase-catalyzed esterification of caproic acid in organic solvent system (2010), J. Mol. Catal. B, 66, 285-293.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of cutinase in Saccharomyces cerevisiae strain SU50 | Fusarium vanettenii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium vanettenii | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 170 | - |
lyophilized cutinase, pH 8.0, 30°C | Fusarium vanettenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenylbutyrate + H2O | - |
Fusarium vanettenii | 4-nitrophenol + butyrate | - |
? |  |
| additional information | cutinase catalyzes esterification of caproic acid in an organic solvent system, alcohol, acid and n-decane are mixed thoroughly in iso-octane before the addition of the lyophilized enzyme, overview. The main kinetic characteristics observed in esterification reaction follow an ordered Ping-Pong Bi-Bi mechanism | Fusarium vanettenii | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Fusarium vanettenii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Fusarium vanettenii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
