Literature summary for 3.1.1.74 extracted from

Eberl, A.; Heumann, S.; Brueckner, T.; Araujo, R.; Cavaco-Paulo, A.; Kaufmann, F.; Kroutil, W.; Guebitz, G.M.
Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules (2009), J. Biotechnol., 143, 207-212.

Search for more literature for 3.1.1.74

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket	Fusarium solani
additional information	no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket	Thermobifida fusca
N,N-diethyl-2-phenylacetamide	increases hydrolysis rates of semi-crystalline poly(ethylene terephthalate) films and fabrics for cutinase. The outer layers of the polymer will be better exposed to the enzyme in the presence of N,N-diethyl-2-phenylacetamide. Overall enhancement in colour depth of 300%	Thermobifida fusca

Organism

Organism	UniProt	Comment	Textmining
Fusarium solani	-	pisi	-
Thermobifida fusca	-	-	-

Storage Stability

Storage Stability	Organism
4°C	Fusarium solani

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxyethyl benzoate + H2O	is hydrolyzed after 24 h of incubation of bisbenzoyloxyethyl terephthalate	Fusarium solani	ethane-1,2-diol + benzoate	-	?
2-hydroxyethyl benzoate + H2O	is hydrolyzed after 24 h of incubation of bisbenzoyloxyethyl terephthalate	Thermobifida fusca	ethane-1,2-diol + benzoate	-	?
bis(2-hydroxyethyl)terephthalate + H2O	fast hydrolysis after treatment for 30 min	Fusarium solani	?	-	?
bis(2-hydroxyethyl)terephthalate + H2O	hydrolysis after treatment for 5 h	Thermobifida fusca	?	-	?
bisbenzoyloxyethyl terephthalate + H2O	-	Fusarium solani	terephthalic acid + mono(2-hydroxyethyl) terephthalate + bis(2-hydroxyethyl)terephthalate + benzoic acid + 2-hydroxyethyl benzoate	-	?
bisbenzoyloxyethyl terephthalate + H2O	-	Thermobifida fusca	terephthalic acid + mono(2-hydroxyethyl) terephthalate + bis(2-hydroxyethyl)terephthalate + benzoic acid + 2-hydroxyethyl benzoate	-	?

Synonyms

Synonyms	Comment	Organism
cutinase	-	Fusarium solani
cutinase	-	Thermobifida fusca

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)