Application | Comment | Organism |
---|---|---|
biotechnology | engineering new cutinase-inspired biocatalysts with tailor-made properties | Aspergillus oryzae |
biotechnology | engineering new cutinase-inspired biocatalysts with tailor-made properties | Fusarium solani |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Fusarium solani |
Crystallization (Comment) | Organism |
---|---|
- |
Fusarium solani |
to 1.75 A resolution. Alpha/beta fold hallmarked by a central beta-sheet of 5 parallel strands surrounded by 10 alpha-helices. An additional disulfide bond and a topologically favored catalytic triad (Ser126, Asp181, and His194), with a continuous and deep groove | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
4-nitrophenyl valerate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae | |
0.00021 | - |
4-nitrophenyl butyrate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae | |
0.00029 | - |
4-nitrophenyl hexanoate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae | |
0.00067 | - |
4-nitrophenyl acetate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.00126 | - |
4-nitrophenyl butyrate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.00148 | - |
4-nitrophenyl valerate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.0015 | - |
4-nitrophenyl hexanoate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.00496 | - |
4-nitrophenyl acetate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Fusarium solani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation, ultrafiltration and on Ni2+ column | Fusarium solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Aspergillus oryzae | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Fusarium solani | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Aspergillus oryzae | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Fusarium solani | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate | - |
Aspergillus oryzae | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate | - |
Fusarium solani | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl valerate | - |
Aspergillus oryzae | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl valerate | - |
Fusarium solani | 4-nitrophenol + pentanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | crystallography | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
cutinase | - |
Aspergillus oryzae |
cutinase | - |
Fusarium solani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
melting temperature. Dramatic loss in activity at 40 °C, 40% drop at 30 °C, continuous decline in activity as a function of increasing temperature | Fusarium solani |
59 | - |
melting temperature. Maintains a high level of activity at 40 °C | Aspergillus oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000043 | - |
4-nitrophenyl butyrate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.00000167 | - |
4-nitrophenyl acetate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae | |
0.0000023 | - |
4-nitrophenyl hexanoate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.00001017 | - |
4-nitrophenyl valerate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.000023 | - |
4-nitrophenyl hexanoate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae | |
0.00004217 | - |
4-nitrophenyl acetate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Fusarium solani | |
0.000053 | - |
4-nitrophenyl valerate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae | |
0.0000582 | - |
4-nitrophenyl butyrate | in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol | Aspergillus oryzae |