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Literature summary for 3.1.1.74 extracted from

  • Wang, G.Y.; Michailides, T.J.; Hammock, B.D.; Lee, Y.M.; Bostock, R.M.
    Molecular cloning, characterization, and expression of a redox-responsive cutinase from Monilinia fructicola (Wint.) Honey (2002), Fungal Genet. Biol., 35, 261-276.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Monilinia fructicola

Protein Variants

Protein Variants Comment Organism
H173L 36% of wild-type activity Monilinia fructicola
S103A 231% of wild-type activity Monilinia fructicola
S103T 38% of wild-type activity Monilinia fructicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.23
-
p-nitrophenylbutanoate 36°C, wild-type Monilinia fructicola
1.45
-
p-nitrophenylbutanoate 36°C, mutant H173L Monilinia fructicola
1.5
-
p-nitrophenylbutanoate 36°C, mutant S103T Monilinia fructicola
1.68
-
p-nitrophenylbutanoate 36°C, mutant S103A Monilinia fructicola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18134
-
x * 20227, precursor protein, x * 18134, mature form, calculated Monilinia fructicola
20227
-
x * 20227, precursor protein, x * 18134, mature form, calculated Monilinia fructicola

Organism

Organism UniProt Comment Textmining
Monilinia fructicola Q8TGB8 enzyme precursor
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information sequence contains a 20 amino-acid secretory signal, one potential N-linked glycosilation site, a protein kinase C phosphorylation site, a lipase active serine and a cutinase active serine Monilinia fructicola

Purification (Commentary)

Purification (Comment) Organism
His-tagged protein, expressed in Pichia pastoris Monilinia fructicola

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenylbutanoate + H2O
-
Monilinia fructicola p-nitrophenol + butanoate
-
?

Subunits

Subunits Comment Organism
? x * 20227, precursor protein, x * 18134, mature form, calculated Monilinia fructicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
p-nitrophenylbutanoate 36°C, mutant H173L Monilinia fructicola
0.25
-
p-nitrophenylbutanoate 36°C, mutant S103T Monilinia fructicola
0.66
-
p-nitrophenylbutanoate 36°C, wild-type Monilinia fructicola
1.5
-
p-nitrophenylbutanoate 36°C, mutant S103A Monilinia fructicola

pI Value

Organism Comment pI Value Maximum pI Value
Monilinia fructicola mature form, calculated
-
8.2
Monilinia fructicola precursor protein, calculated
-
8.4