Literature summary for 3.1.1.74 extracted from
Calado, C.R.C.; Taipa, M.A.; Cabral, J.M.S.; Fonseca, L.P.
Optimisation of culture conditions and characterisation of cutinase produced by recombinant Saccharomyces cerevisiae (2002), Enzyme Microb. Technol., 31, 161-170.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
a high enzyme production, high specific enzyme activity, and high enzyme yield are obtained upon expression with a 5% air saturation of oxygen. At low dissolved oxygen concentration, enzyme yield and specific activity increase with increase of culture pH-value from 5.25 to 6.25 |
Fusarium vanettenii |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
22800 |
- |
x * 22800, x * 24900, two differently glycosylated forms, SDS-PAGE |
Fusarium vanettenii |
24900 |
- |
x * 22800, x * 24900, two differently glycosylated forms, SDS-PAGE |
Fusarium vanettenii |
Organism
Organism |
UniProt |
Comment |
Textmining |
Fusarium vanettenii |
- |
expression in Saccharomyces cerevisiae |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
glycoprotein |
two differently glycosylated forms of 22800 and 24900 Da |
Fusarium vanettenii |
Storage Stability
Storage Stability |
Organism |
22°C, high stability |
Fusarium vanettenii |
Subunits
Subunits |
Comment |
Organism |
? |
x * 22800, x * 24900, two differently glycosylated forms, SDS-PAGE |
Fusarium vanettenii |