Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.73 extracted from

  • Antonopoulou, I.; Hunt, C.; Cerullo, G.; Varriale, S.; Gerogianni, A.; Faraco, V.; Rova, U.; Christakopoulos, P.
    Tailoring the specificity of the type C feruloyl esterase FoFaeC from Fusarium oxysporum towards methyl sinapate by rational redesign based on small molecule docking simulations (2018), PLoS ONE, 13, e0198127 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Fusarium oxysporum

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure and docking of substrates Fusarium oxysporum

Protein Variants

Protein Variants Comment Organism
additional information introduction of mutations to provide hydrophobic environment for increased hydrolysis of sinapate substrates Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.331
-
 methyl ferulate wild-type, pH 6.0, 37°C Fusarium oxysporum
0.424
-
 methyl sinapate wild-type, pH 6.0, 37°C Fusarium oxysporum
0.649
-
 methyl caffeate wild-type, pH 6.0, 37°C Fusarium oxysporum
0.799
-
 methyl p-coumarate wild-type, pH 6.0, 37°C Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum A0A1D3S5H0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl caffeate + H2O 85% of the activity with methyl p-coumarate Fusarium oxysporum methanol + caffeate
-
 ?
methyl ferulate + H2O 54% of the activity with methyl p-coumarate Fusarium oxysporum methanol + ferulate
-
 ?
methyl p-coumarate + H2O
-
 Fusarium oxysporum methanol + p-coumarate
-
 ?
methyl sinapate + H2O 2.8% of the activity with methyl p-coumarate Fusarium oxysporum methanol + sinapate
-
 ?

Synonyms

Synonyms Comment Organism
FaeC
-
 Fusarium oxysporum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
 methyl sinapate wild-type, pH 6.0, 37°C Fusarium oxysporum
232
-
 methyl ferulate wild-type, pH 6.0, 37°C Fusarium oxysporum
438
-
 methyl caffeate wild-type, pH 6.0, 37°C Fusarium oxysporum
546
-
 methyl p-coumarate wild-type, pH 6.0, 37°C Fusarium oxysporum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
40
-
 methyl sinapate wild-type, pH 6.0, 37°C Fusarium oxysporum
675
-
 methyl caffeate wild-type, pH 6.0, 37°C Fusarium oxysporum
682
-
 methyl p-coumarate wild-type, pH 6.0, 37°C Fusarium oxysporum
744
-
 methyl ferulate wild-type, pH 6.0, 37°C Fusarium oxysporum