Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, 83% of initial activity | Aspergillus ochraceus | |
Cu2+ | 1 mM, 55% of initial activity | Aspergillus ochraceus | |
Hg2+ | 1 mM, 10% of initial activity | Aspergillus ochraceus | |
PMSF | 1 mM, no residual activity | Aspergillus ochraceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
methyl caffeate | pH 7.2, 30°C | Aspergillus ochraceus | |
0.58 | - |
methyl p-coumarate | pH 7.2, 30°C | Aspergillus ochraceus | |
0.64 | - |
methyl ferulate | pH 7.2, 30°C | Aspergillus ochraceus | |
1.11 | - |
methyl sinapinate | pH 7.2, 30°C | Aspergillus ochraceus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 209% of initial activity | Aspergillus ochraceus | |
Mg2+ | 1 mM, 177% of initial activity | Aspergillus ochraceus | |
Mn2+ | 1 mM, 143% of initial activity | Aspergillus ochraceus | |
Zn2+ | 1 mM, 112% of initial activity | Aspergillus ochraceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26500 | - |
gel filtration | Aspergillus ochraceus |
Organic Solvent | Comment | Organism |
---|---|---|
Butanol | maximum initial synthetic rate of 2.8 nmol/min for transesterification reaction to yiled butyl ferulate is reached at 25% (v/v) butanol, using an isooctane:butanol:water relation of 73:25:2 | Aspergillus ochraceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus ochraceus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus ochraceus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57.9 | - |
substrate methyl ferulate, pH 7.2, 30°C | Aspergillus ochraceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl caffeate + H2O | - |
Aspergillus ochraceus | methanol + caffeate | - |
? | |
methyl ferulate + butanol | - |
Aspergillus ochraceus | methanol + butyl ferulate | - |
? | |
methyl ferulate + H2O | - |
Aspergillus ochraceus | methanol + ferulate | - |
? | |
methyl p-coumarate + H2O | - |
Aspergillus ochraceus | methanol + p-coumarate | - |
? | |
methyl sinapinate + H2O | - |
Aspergillus ochraceus | methanol + sinapinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34600, SDS-PAGE | Aspergillus ochraceus |
Synonyms | Comment | Organism |
---|---|---|
FaeC | - |
Aspergillus ochraceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus ochraceus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2306 | - |
methyl caffeate | pH 7.2, 30°C | Aspergillus ochraceus | |
2306 | - |
methyl sinapinate | pH 7.2, 30°C | Aspergillus ochraceus | |
2710 | - |
methyl p-coumarate | pH 7.2, 30°C | Aspergillus ochraceus | |
3344 | - |
methyl ferulate | pH 7.2, 30°C | Aspergillus ochraceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Aspergillus ochraceus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | - |
40% of maximum activity | Aspergillus ochraceus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2078 | - |
methyl sinapinate | pH 7.2, 30°C | Aspergillus ochraceus | |
4352 | - |
methyl caffeate | pH 7.2, 30°C | Aspergillus ochraceus | |
4673 | - |
methyl p-coumarate | pH 7.2, 30°C | Aspergillus ochraceus | |
5226 | - |
methyl ferulate | pH 7.2, 30°C | Aspergillus ochraceus |