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Literature summary for 3.1.1.73 extracted from
- Feruloyl esterase immobilization in mesoporous silica particles and characterization in hydrolysis and transesterification (2018), BMC Biochem., 19, 0001 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme immobilized on mesoporous silica by physical adsorption shows more than 3fold variation in the transesterification/hydrolysis products molar ratio depending on the reaction buffer used and its pH. Hydrolysis is the dominant activity of the enzyme. Km is not affected by the immobilization but kcat is reduced 10fold. The immobilized enzyme retains more than 20% of its activity after ten cycles of transesterification reaction | rumen microorganism |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
methyl ferulate | hydrolysis, free enzyme, pH not specified in the publication, 40°C | rumen microorganism |  |
| 0.43 | - |
methyl ferulate | hydrolysis, immobilized enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 30.9 | - |
methyl ferulate | transesterification, immobilized enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 36 | - |
methyl ferulate | transesterification, free enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| rumen microorganism | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
rumen microorganism | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl ferulate + butanol | - |
rumen microorganism | methanol + butyl ferulate | - |
? | |
| methyl ferulate + H2O | - |
rumen microorganism | methanol + ferulate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, 25% residual activity for free enzyme, 60% for immobilized enzyme | rumen microorganism |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
methyl ferulate | transesterification, immobilized enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 0.14 | - |
methyl ferulate | transesterification, free enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 6.47 | - |
methyl ferulate | hydrolysis, immobilized enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 31.4 | - |
methyl ferulate | hydrolysis, free enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
immobilized enzyme | rumen microorganism |
| 7.5 | - |
free enzyme | rumen microorganism |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00035 | - |
methyl ferulate | transesterification, immobilized enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 0.0038 | - |
methyl ferulate | transesterification, free enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 15 | - |
methyl ferulate | hydrolysis, immobilized enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
| 72.9 | - |
methyl ferulate | hydrolysis, free enzyme, pH not specified in the publication, 40°C | rumen microorganism | |
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