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Literature summary for 3.1.1.73 extracted from

  • Long, L.; Zhao, H.; Ding, D.; Xu, M.; Ding, S.
    Heterologous expression of two Aspergillus niger feruloyl esterases in Trichoderma reesei for the production of ferulic acid from wheat bran (2018), Bioprocess Biosyst. Eng., 41, 593-601 .
    View publication on PubMed

Application

Application Comment Organism
degradation overexpression in Hypocrea jecorina leads to a high level of feruloyl esterase produced under solid-state fermentation. The recombinant fungal enzyme system can release 52.2% of total ferulic acids from destarched wheat bran Aspergillus niger
degradation overexpression in Hypocrea jecorina leads to a high level of feruloyl esterase produced under solid-state fermentation. The recombinant fungal enzyme system can release 62.9% of total ferulic acids from destarched wheat bran Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression in Hypocrea jecorina Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A0A2I6U1R0 isoform FaeB
-
Aspergillus niger A0A2I6U1R5 isoform FaeA
-
Aspergillus niger 0913 A0A2I6U1R0 isoform FaeB
-
Aspergillus niger 0913 A0A2I6U1R5 isoform FaeA
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.1
-
pH 6.0, 35°C Aspergillus niger
20.7
-
pH 5.0, 50°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl ferulate + H2O
-
Aspergillus niger methanol + ferulate
-
?
methyl ferulate + H2O
-
Aspergillus niger 0913 methanol + ferulate
-
?

Subunits

Subunits Comment Organism
? x * 56420, calculated from sequence including His-tag Aspergillus niger

Synonyms

Synonyms Comment Organism
FAEA
-
Aspergillus niger
FAEB
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Aspergillus niger
50
-
-
Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus niger
6
-
-
Aspergillus niger