Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.73 extracted from

  • Varriale, S.; Cerullo, G.; Antonopoulou, I.; Christakopoulos, P.; Rova, U.; Tron, T.; Faure, R.; Juetten, P.; Piechot, A.; Bras, J.L.A.; Fontes, C.M.G.A.; Faraco, V.
    Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis (2018), Appl. Microbiol. Biotechnol., 102, 5185-5196 .
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Thermothelomyces thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular docking of methyl hydroxycinnamates to wild-type and mutant structures Thermothelomyces thermophilus

Protein Variants

Protein Variants Comment Organism
F251L/H105Y mutant displays improved activity and thermostability Thermothelomyces thermophilus
G49A mutant displays improved activity and thermostability Thermothelomyces thermophilus
G49D mutant displays improved activity and thermostability Thermothelomyces thermophilus
H105Y mutant displays improved activity and thermostability Thermothelomyces thermophilus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone wild-type, 25% acetone, v/v, 1 h, 67% residual activity Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2QND5
-
-
Thermothelomyces thermophilus DSM 1799 G2QND5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrocatechol-1-yl ferulate + H2O
-
 Thermothelomyces thermophilus 4-nitrocatechol + ferulate
-
 ?
4-nitrocatechol-1-yl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 4-nitrocatechol + ferulate
-
 ?
4-nitrophenyl ferulate + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + ferulate
-
 ?
4-nitrophenyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 4-nitrophenol + ferulate
-
 ?

Synonyms

Synonyms Comment Organism
Fae1A
-
 Thermothelomyces thermophilus
feruloyl esterase B
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 wild-type, 1 h, 10.8%, mutant H105Y 34.3%, mutant F251L/H105Y 21.4%, mutant G49D 46.2%, mutant G49A 37.4% residual activity, respectively Thermothelomyces thermophilus