Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.73 extracted from

  • Koseki, T.; Handa, H.; Watanabe, Y.; Ohtsuka, M.; Shiono, Y.
    An unusual feruloyl esterase from Aspergillus oryzae two tryptophan residues play a crucial role for the activity (2016), J. Mol. Catal. B, 133, S560-S568 .
No PubMed abstract available
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
W142F contrary to wild type, mutant is active against methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid and exhibits enhanced production of ferulic acid from wheat arabinoxylan Aspergillus oryzae
W144Y contrary to wild type, mutant is active against methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid and exhibits enhanced production of ferulic acid from wheat arabinoxylan Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 methyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.044
-
 ethyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.05
-
 methyl p-coumarate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.057
-
 methyl caffeate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.067
-
 methyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.086
-
 ethyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.1
-
 methyl sinapinate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.11
-
 methyl p-coumarate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.12
-
 methyl caffeate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.15
-
 methyl sinapinate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.44
-
 alpha-naphthyl propionate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.45
-
 alpha-naphthyl propionate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.58
-
 alpha-naphthyl propionate wild-type, pH 7.0, 37°C Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular recombinant protein Aspergillus oryzae
-
-

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q2U7D3
-
-
Aspergillus oryzae ATCC 42149 Q2U7D3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-naphthyl propionate + H2O
-
 Aspergillus oryzae alpha-naphthol + propionate
-
 ?
alpha-naphthyl propionate + H2O
-
 Aspergillus oryzae ATCC 42149 alpha-naphthol + propionate
-
 ?
arabinoxylan + H2O
-
 Aspergillus oryzae ferulic acid + ?
-
 ?
arabinoxylan + H2O
-
 Aspergillus oryzae ATCC 42149 ferulic acid + ?
-
 ?
ethyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae ethanol + ferulate
-
 ?
ethyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae ATCC 42149 ethanol + ferulate
-
 ?
methyl caffeate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + caffeate
-
 ?
methyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + ferulate
-
 ?
methyl ferulate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae ATCC 42149 methanol + ferulate
-
 ?
methyl p-coumarate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + p-coumarate
-
 ?
methyl sinapinate + H2O no substrate of wild-type, but substrate of mutants W144Y and W142F Aspergillus oryzae methanol + sinapinate
-
 ?
additional information wild-type rAoFaeD fails to cleave the methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid Aspergillus oryzae ?
-
 ?
additional information wild-type rAoFaeD fails to cleave the methyl esters of ferulic acid, p-coumaric acid, caffeic acid, and sinapic acid and ethyl ester of ferulic acid Aspergillus oryzae ATCC 42149 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
AO090701000884
-
 Aspergillus oryzae
FaeD
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 methyl caffeate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.23
-
 methyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.31
-
 methyl caffeate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.35
-
 methyl p-coumarate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
0.51
-
 methyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.57
-
 methyl p-coumarate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
0.68
-
 methyl sinapinate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
1.78
-
 methyl sinapinate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.79
-
 alpha-naphthyl propionate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
5.74
-
 alpha-naphthyl propionate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
5.8
-
 alpha-naphthyl propionate wild-type, pH 7.0, 37°C Aspergillus oryzae
71.6
-
 ethyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
92.1
-
 ethyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10
-
 Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6
-
 methyl caffeate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
3.2
-
 methyl caffeate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
5.5
-
 methyl p-coumarate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
6.2
-
 methyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
6.7
-
 methyl sinapinate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
6.9
-
 methyl p-coumarate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
7.7
-
 methyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
8.6
-
 alpha-naphthyl propionate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
10
-
 alpha-naphthyl propionate wild-type, pH 7.0, 37°C Aspergillus oryzae
11.9
-
 methyl sinapinate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
12.7
-
 alpha-naphthyl propionate mutant W142F, pH 7.0, 37°C Aspergillus oryzae
1071
-
 ethyl ferulate mutant W144Y, pH 7.0, 37°C Aspergillus oryzae
1627
-
 ethyl ferulate mutant W142F, pH 7.0, 37°C Aspergillus oryzae