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Literature summary for 3.1.1.73 extracted from

  • Schaer, A.; Sprecher, I.; Topakas, E.; Faulds, C.B.; Nystroem, L.
    Hydrolysis of nonpolar n-alkyl ferulates by feruloyl esterases (2016), J. Agric. Food Chem., 64, 8549-8554 .
    View publication on PubMed
Search for more literature for 3.1.1.73

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 n-decyl ferulate pH 7.0, 40°C rumen bacterium
0.008
-
 n-decyl ferulate pH 6.0, 40°C Aspergillus niger
0.01
-
 n-hexyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
0.027
-
 n-propyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
0.027
-
 n-hexyl ferulate pH 7.0, 40°C rumen bacterium
0.029
-
 n-hexyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
0.04
-
 n-hexyl ferulate pH 6.0, 40°C Aspergillus niger
0.048
-
 ethyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
0.051
-
 methyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
0.081
-
 n-propyl ferulate pH 7.0, 40°C rumen bacterium
0.125
-
 n-decyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
0.134
-
 methyl ferulate pH 7.0, 40°C rumen bacterium
0.149
-
 ethyl ferulate pH 7.0, 40°C rumen bacterium
0.245
-
 n-propyl ferulate pH 6.0, 40°C Aspergillus niger
0.611
-
 ethyl ferulate pH 6.0, 40°C Aspergillus niger
1.123
-
 methyl ferulate pH 6.0, 40°C Aspergillus niger
1.237
-
 n-propyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
2.472
-
 methyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
2.578
-
 ethyl ferulate pH 7.0, 40°C Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Aspergillus niger O42807
-
-
rumen bacterium
-
-
-
Thermothelomyces thermophilus G2QND5
-
-
Thermothelomyces thermophilus DSM 1799 G2QND5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 rumen bacterium
-
commercial preparation
-
 Acetivibrio thermocellus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl ferulate + H2O
-
 rumen bacterium ferulate + ethanol
-
 ?
ethyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + ethanol
-
 ?
ethyl ferulate + H2O
-
 Aspergillus niger ferulate + ethanol
-
 ?
ethyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + ethanol
-
 ?
ethyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + ethanol
-
 ?
methyl ferulate + H2O
-
 rumen bacterium ferulate + methanol
-
 ?
methyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + methanol
-
 ?
methyl ferulate + H2O
-
 Aspergillus niger ferulate + methanol
-
 ?
methyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + methanol
-
 ?
methyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + methanol
-
 ?
n-decyl ferulate + H2O
-
 rumen bacterium ferulate + n-decanol
-
 ?
n-decyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + n-decanol
-
 ?
n-decyl ferulate + H2O
-
 Aspergillus niger ferulate + n-decanol
-
 ?
n-hexyl ferulate + H2O
-
 rumen bacterium ferulate + n-hexanol
-
 ?
n-hexyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + n-hexanol
-
 ?
n-hexyl ferulate + H2O
-
 Aspergillus niger ferulate + n-hexanol
-
 ?
n-hexyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + n-hexanol
-
 ?
n-hexyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + n-hexanol
-
 ?
n-propyl ferulate + H2O
-
 rumen bacterium ferulate + n-propanol
-
 ?
n-propyl ferulate + H2O
-
 Acetivibrio thermocellus ferulate + n-propanol
-
 ?
n-propyl ferulate + H2O
-
 Aspergillus niger ferulate + n-propanol
-
 ?
n-propyl ferulate + H2O
-
 Thermothelomyces thermophilus ferulate + n-propanol
-
 ?
n-propyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 ferulate + n-propanol
-
 ?

Synonyms

Synonyms Comment Organism
Fae1A
-
 Thermothelomyces thermophilus
FAEA
-
 Aspergillus niger
feruloyl esterase B
-
 Thermothelomyces thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 n-hexyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
0.4
-
 n-decyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
2.6
-
 n-decyl ferulate pH 7.0, 40°C rumen bacterium
3.2
-
 n-propyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
4.6
-
 n-decyl ferulate pH 6.0, 40°C Aspergillus niger
5.7
-
 ethyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
6.1
-
 n-hexyl ferulate pH 7.0, 40°C rumen bacterium
8
-
 methyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
8.8
-
 methyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
8.9
-
 n-hexyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
9.8
-
 n-hexyl ferulate pH 6.0, 40°C Aspergillus niger
11.2
-
 ethyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
12.1
-
 n-propyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
29.4
-
 ethyl ferulate pH 6.0, 40°C Aspergillus niger
30.7
-
 ethyl ferulate pH 7.0, 40°C rumen bacterium
31.7
-
 n-propyl ferulate pH 7.0, 40°C rumen bacterium
32.9
-
 methyl ferulate pH 6.0, 40°C Aspergillus niger
33.5
-
 methyl ferulate pH 7.0, 40°C rumen bacterium
44.6
-
 n-propyl ferulate pH 6.0, 40°C Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2
-
 ethyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
2.6
-
 n-propyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.2
-
 methyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
3.2
-
 n-decyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
5.6
-
 n-hexyl ferulate pH 7.0, 40°C Acetivibrio thermocellus
29
-
 methyl ferulate pH 6.0, 40°C Aspergillus niger
48
-
 ethyl ferulate pH 6.0, 40°C Aspergillus niger
173
-
 methyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
182
-
 n-propyl ferulate pH 6.0, 40°C Aspergillus niger
206
-
 ethyl ferulate pH 7.0, 40°C rumen bacterium
225
-
 n-hexyl ferulate pH 7.0, 40°C rumen bacterium
236
-
 ethyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
243
-
 n-hexyl ferulate pH 6.0, 40°C Aspergillus niger
250
-
 methyl ferulate pH 7.0, 40°C rumen bacterium
391
-
 n-propyl ferulate pH 7.0, 40°C rumen bacterium
452
-
 n-propyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus
547
-
 n-decyl ferulate pH 6.0, 40°C Aspergillus niger
780
-
 n-decyl ferulate pH 7.0, 40°C rumen bacterium
906
-
 n-hexyl ferulate pH 6.0, 40°C Thermothelomyces thermophilus