Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 5 mM, 90% residual activity | Lactarius hatsudake | |
Ca2+ | 5 mM, 91% residual activity | Lactarius hatsudake | |
Cd2+ | 5 mM, 84% residual activity | Lactarius hatsudake | |
Cu2+ | 5 mM, 88% residual activity | Lactarius hatsudake | |
Fe2+ | 5 mM, no residual activity | Lactarius hatsudake | |
Hg2+ | 5 mM, no resiudal activity | Lactarius hatsudake | |
Mn2+ | 5 mM, 83% residual activity | Lactarius hatsudake | |
additional information | not inhibiting/activating: K+ or Zn2+ at 5 mM | Lactarius hatsudake |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, 114% of initial activity | Lactarius hatsudake |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Lactarius hatsudake |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactarius hatsudake | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactarius hatsudake |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
substrate methyl p-coumarate, pH 4.0, 30°C | Lactarius hatsudake |
9.66 | - |
substrate methyl ferulate, pH 4.0, 30°C | Lactarius hatsudake |
13.14 | - |
substrate methyl caffeate, pH 4.0, 30°C | Lactarius hatsudake |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
destarched wheat bran + H2O | - |
Lactarius hatsudake | ferulic acid + ? | - |
? | |
methyl caffeate + H2O | - |
Lactarius hatsudake | caffeate + methanol | - |
? | |
methyl ferulate + H2O | - |
Lactarius hatsudake | ferulate + methanol | - |
? | |
methyl p-coumarate + H2O | - |
Lactarius hatsudake | methanol + p-coumarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Lactarius hatsudake |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lactarius hatsudake |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
66% of maximum activity | Lactarius hatsudake |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Lactarius hatsudake |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | - |
71% of maximum activity | Lactarius hatsudake |
6 | - |
completely inactive | Lactarius hatsudake |