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Literature summary for 3.1.1.73 extracted from

  • Wang, L.; Li, Z.; Zhu, M.; Meng, L.; Wang, H.; Ng, T.B.
    An acidic feruloyl esterase from the mushroom Lactarius hatsudake A potential animal feed supplement (2016), Int. J. Biol. Macromol., 93, 290-295 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 5 mM, 90% residual activity Lactarius hatsudake
Ca2+ 5 mM, 91% residual activity Lactarius hatsudake
Cd2+ 5 mM, 84% residual activity Lactarius hatsudake
Cu2+ 5 mM, 88% residual activity Lactarius hatsudake
Fe2+ 5 mM, no residual activity Lactarius hatsudake
Hg2+ 5 mM, no resiudal activity Lactarius hatsudake
Mn2+ 5 mM, 83% residual activity Lactarius hatsudake
additional information not inhibiting/activating: K+ or Zn2+ at 5 mM Lactarius hatsudake

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM, 114% of initial activity Lactarius hatsudake

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
gel filtration Lactarius hatsudake

Organism

Organism UniProt Comment Textmining
Lactarius hatsudake
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactarius hatsudake

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
substrate methyl p-coumarate, pH 4.0, 30°C Lactarius hatsudake
9.66
-
substrate methyl ferulate, pH 4.0, 30°C Lactarius hatsudake
13.14
-
substrate methyl caffeate, pH 4.0, 30°C Lactarius hatsudake

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
destarched wheat bran + H2O
-
Lactarius hatsudake ferulic acid + ?
-
?
methyl caffeate + H2O
-
Lactarius hatsudake caffeate + methanol
-
?
methyl ferulate + H2O
-
Lactarius hatsudake ferulate + methanol
-
?
methyl p-coumarate + H2O
-
Lactarius hatsudake methanol + p-coumarate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Lactarius hatsudake

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Lactarius hatsudake

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
66% of maximum activity Lactarius hatsudake

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Lactarius hatsudake

pH Range

pH Minimum pH Maximum Comment Organism
3
-
71% of maximum activity Lactarius hatsudake
6
-
completely inactive Lactarius hatsudake