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Literature summary for 3.1.1.73 extracted from

  • Kelle, S.; Nieter, A.; Krings, U.; Zelena, K.; Linke, D.; Berger, R.
    Heterologous production of a feruloyl esterase from Pleurotus sapidus synthesizing feruloyl-saccharide esters (2015), Biotechnol. Appl. Biochem., 63, 852-862.
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Pichia pastoris Pleurotus sapidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.227
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus
0.427
-
 ferulic acid methyl ester pH 5.0, 37°C Pleurotus sapidus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pleurotus sapidus
-
-

Organism

Organism UniProt Comment Textmining
Pleurotus sapidus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1397
-
 substrate 5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C Pleurotus sapidus
1681
-
 substrate beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C Pleurotus sapidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-O-trans-feruloyl-L-arabinofuranose + H2O 83% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + L-arabinofuranose
-
 ?
alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose 48% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose
-
 ?
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O
-
 Pleurotus sapidus ferulic acid + beta-D-xylopyranosyl-(1->2)-L-arabinofuranose
-
 ?
ferulic acid ethyl ester + H2O 62% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + ethanol
-
 ?
ferulic acid methyl ester + H2O 61% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + methanol
-
 ?
additional information enzyme additionally catalyzes transferulation using methyl ferulate as a ferulic acid donor and glucose, fructose, or galactose as acceptor, leading to two ferulated monosaccharide anomers each Pleurotus sapidus ?
-
 ?
sinapinic acid methyl ester + H2O 22% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus sinapinic acid + methanol
-
 ?
wheat bran + H2O
-
 Pleurotus sapidus ferulic acid + ?
-
 ?

Synonyms

Synonyms Comment Organism
Est1
-
 Pleurotus sapidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pleurotus sapidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 stable for 1 h Pleurotus sapidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 ferulic acid methyl ester pH 5.0, 37°C Pleurotus sapidus
1.96
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pleurotus sapidus

pH Range

pH Minimum pH Maximum Comment Organism
3 8 no activity below or above Pleurotus sapidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.81
-
 ferulic acid methyl ester pH 5.0, 37°C Pleurotus sapidus
8.64
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus