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Literature summary for 3.1.1.73 extracted from
- Purification and biochemical characterization of feruloyl esterases from Aspergillus terreus MTCC 11096 (2013), Biotechnol. Prog., 29, 924-932.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Aspergillus terreus |  |
| Fe2+ | - |
Aspergillus terreus | |
| Fe3+ | - |
Aspergillus terreus | |
| Hg2+ | complete inhibition at 1 mM; complete inhibition at 1 mM | Aspergillus terreus | |
| K+ | - |
Aspergillus terreus | |
| Mg2+ | - |
Aspergillus terreus | |
| PMSF | complete inhibition at 1 mM; complete inhibition at 1 mM | Aspergillus terreus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+ has no significant effect on the enzyme activity | Aspergillus terreus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, SDS-PAGE | Aspergillus terreus |
| 36000 | - |
x * 36000, SDS-PAGE | Aspergillus terreus |
| 76000 | - |
x * 76000, SDS-PAGE | Aspergillus terreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-2 | - |
| Aspergillus terreus | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-3 | - |
| Aspergillus terreus | Q0CBM7 | isolated from the soils of agricultural fields cultivating sweet sorghum, type A isozyme AtFAE-1 | - |
| Aspergillus terreus MTCC 11096 | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-2 | - |
| Aspergillus terreus MTCC 11096 | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-3 | - |
| Aspergillus terreus MTCC 11096 | Q0CBM7 | isolated from the soils of agricultural fields cultivating sweet sorghum, type A isozyme AtFAE-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozyme AtFAE-1 67fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography | Aspergillus terreus |
| native isozyme AtFAE-2 58fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration | Aspergillus terreus |
| native isozyme AtFAE-3 56fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration | Aspergillus terreus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 263.3 | - |
purified isozyme, pH 5.0, 40°C | Aspergillus terreus |
| 275.5 | - |
purified isozyme, pH 5.0, 40°C | Aspergillus terreus |
| 317.7 | - |
purified isozyme, pH 5.0, 40°C | Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl ferulate + H2O | - |
Aspergillus terreus | methanol + ferulate | - |
? | |
| methyl ferulate + H2O | - |
Aspergillus terreus MTCC 11096 | methanol + ferulate | - |
? | |
| additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-1 for diverse ester substrates, overview | Aspergillus terreus | ? | - |
? | |
| additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-2 for diverse ester substrates, overview | Aspergillus terreus | ? | - |
? | |
| additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-3 for diverse ester substrates, overview | Aspergillus terreus | ? | - |
? | |
| additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-2 for diverse ester substrates, overview | Aspergillus terreus MTCC 11096 | ? | - |
? | |
| additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-3 for diverse ester substrates, overview | Aspergillus terreus MTCC 11096 | ? | - |
? | |
| additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-1 for diverse ester substrates, overview | Aspergillus terreus MTCC 11096 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, SDS-PAGE | Aspergillus terreus |
| ? | x * 36000, SDS-PAGE | Aspergillus terreus |
| ? | x * 23000, SDS-PAGE | Aspergillus terreus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtFAE-1 | - |
Aspergillus terreus |
| AtFAE-2 | - |
Aspergillus terreus |
| AtFAE-3 | - |
Aspergillus terreus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus terreus |
| 50 | - |
- |
Aspergillus terreus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified isozyme AtFAE-2, 4 h incubation at pH 3.0-8.0, over 50% activity remaining | Aspergillus terreus |
| 40 | - |
purified isozyme AtFAE-3, 4 h incubation at pH 3.0-8.0, over 50% activity remaining | Aspergillus terreus |
| 50 | - |
purified isozyme AtFAE-1, 4 h incubation at pH 3.0-8.0, over 50% activity remaining | Aspergillus terreus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus terreus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | over 50% of maximal activity within this range | Aspergillus terreus |
| 5 | 6 | over 50% of maximal activity within this range | Aspergillus terreus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | purified isozyme AtFAE-1, 4 h incubation at 50°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions | Aspergillus terreus |
| 3 | 8 | purified isozyme AtFAE-2, 4 h incubation at 40°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions | Aspergillus terreus |
| 3 | 8 | purified isozyme AtFAE-3, 4 h incubation at 50°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions | Aspergillus terreus |
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