Literature summary for 3.1.1.73 extracted from

Badhan, A.; Jin, L.; Wang, Y.; Han, S.; Kowalczys, K.; Brown, D.C.; Ayala, C.J.; Latoszek-Green, M.; Miki, B.; Tsang, A.; McAllister, T.
Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility (2014), Biotechnol. Biofuels, 7, 39.

Cloned(Commentary)

Cloned (Comment)	Organism
gene faeB, subcloned into pEACH vector without its own N-terminal signal peptide coding region, but containing different signaling peptides to target the enzyme to the apoplast, chloroplast, endoplasmic reticulum, and vacuole. Four constructs harboring faeB are transiently expressed in Nicotiana tabacum leaves, with the enzyme accumulating at high levels in all target sites, except chloroplast. Stable expression in Medicago sativa using Agrobacterium tumefaciens strain LBA4404 transformation method	Aspergillus niger

Cloned (Comment)

Organism

gene faeB, subcloned into pEACH vector without its own N-terminal signal peptide coding region, but containing different signaling peptides to target the enzyme to the apoplast, chloroplast, endoplasmic reticulum, and vacuole. Four constructs harboring faeB are transiently expressed in Nicotiana tabacum leaves, with the enzyme accumulating at high levels in all target sites, except chloroplast. Stable expression in Medicago sativa using Agrobacterium tumefaciens strain LBA4404 transformation method

Aspergillus niger

Protein Variants

Protein Variants	Comment	Organism
additional information	transgenic alflafa plants expressing the enzyme possess modified cell wall morphology and composition with a reduction in ester linkages and elevated lignin content, they are more recalcitrant to digestion by mixed ruminal microorganisms, phenotypes, detailed overview	Aspergillus niger

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	18 amino acids in the Nterminus function as a signal peptide that aides in the secretion of the enzyme	Aspergillus niger	-	-

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	Q8WZI8	-	-
Aspergillus niger	Q8WZI8	gene faeB	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ethyl ferulate + H2O	-	Aspergillus niger	ethanol + ferulate	-	?

Synonyms

Synonyms	Comment	Organism
FAEB	-	Aspergillus niger
ferulic acid esterase	-	Aspergillus niger
feruloyl esterase B	-	Aspergillus niger
type B ferulic acid esterase	-	Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Aspergillus niger

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Aspergillus niger

General Information

General Information	Comment	Organism
physiological function	expression in Medicago sativa and Nicotiana tabacum using different signaling peptides to target type B ferulic acid esterase proteins to the apoplast, chloroplast, endoplasmic reticulum and vacuole. In Nicotiana leaves, the protein accumulates at high levels in all target sites, except chloroplast. Stable transformed lines of alfalfa possess modified cell wall morphology and composition with a reduction in ester linkages and elevated lignin content. These are more recalcitrant to digestion by mixed ruminal microorganisms. Delignification by alkaline peroxide treatment followed by exposure to a commercial cellulase mixture results in higher glucose release from transgenic lines as compared to the control line	Aspergillus niger