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Literature summary for 3.1.1.73 extracted from

  • Zhang, S.B.; Pei, X.Q.; Wu, Z.L.
    Multiple amino acid substitutions significantly improve the thermostability of feruloyl esterase A from Aspergillus niger (2012), Biores. Technol., 117, 140-147.
    View publication on PubMed
Search for more literature for 3.1.1.73

Protein Variants

Protein Variants Comment Organism
A140T random mutagenesis, the mutant shows 2.4fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
C235S random mutagenesis, the mutant shows 3.2fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
D93G site-directed mutagenesis, the mutant shows increased thermostability compared to the wild-type enzyme Aspergillus niger
D93G/S187F site-directed mutagenesis, the mutant shows 10fold increased activity compared to the wild-type enzyme Aspergillus niger
G69A random mutagenesis, the mutant shows unaltered thermostability compared to the mutant D93G/S187F Aspergillus niger
K37I random mutagenesis, the mutant shows 2.5fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
K37I/G69A random mutagenesis, the mutant shows 2.5fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
L14F random mutagenesis, the mutant shows 2.0fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R the mutant shows altered kinetics compared to the mutant D93G/S187F Aspergillus niger
L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R/C235S the mutant shows altered kinetics compared to the mutant D93G/S187F Aspergillus niger
additional information identification of mutations beneficial to the thermostability of the nezyme, screening a random mutagenesis library constructed in Pichia pastoris Aspergillus niger
Q121H random mutagenesis, the mutant shows 1.8fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
Q177H random mutagenesis, the mutant shows 2.0fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
Q185R random mutagenesis, the mutant shows 2.5fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
S163T random mutagenesis, the mutant shows 3.6fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
S187F site-directed mutagenesis, the mutant shows increased thermostability compared to the wild-type enzyme Aspergillus niger
T35I random mutagenesis, the mutant shows 3.4fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
T57I random mutagenesis, the mutant shows 1.4fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
T57I/V178A random mutagenesis, the mutant 2.3fold shows increased thermostability compared to the mutant D93G/S187F Aspergillus niger
T63I random mutagenesis, the mutant shows 2.5fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger
V178A random mutagenesis, the mutant shows 1.6fold increased thermostability compared to the mutant D93G/S187F Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.65
-
 methyl ferulate mutant D93G/S187F, pH 6.4, 40°C Aspergillus niger
3.96
-
 methyl ferulate mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R, pH 6.4, 40°C Aspergillus niger
4.08
-
 methyl ferulate mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R/C235S, pH 6.4, 40°C Aspergillus niger
6.34
-
 methyl ferulate mutant C235S, pH 6.4, 40°C Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger O42807
-
-
Aspergillus niger CIB 423.1 O42807
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl ferulate + H2O
-
 Aspergillus niger methanol + ferulate
-
 ?
methyl ferulate + H2O
-
 Aspergillus niger CIB 423.1 methanol + ferulate
-
 ?

Synonyms

Synonyms Comment Organism
AnFaeA
-
 Aspergillus niger
feruloyl esterase A
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 mutations T57I, V178A, and K37I are beneficial to the thermostability of AnFaeA with 1.5-3fold improvement in half-life Aspergillus niger
55
-
 half-life of mutant D93G/S187F is 4.7 min, half-lives of mutants with further increased thermostability, best is mutant S163T with a half-life of 16.9 min, overview Aspergillus niger
63
-
 30 min, mutant D93G/S187F shows a loss of around 70-80% activity, mutant C235S shows a loss of about 10% activity Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.79
-
 methyl ferulate mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R/C235S, pH 6.4, 40°C Aspergillus niger
0.89
-
 methyl ferulate mutant C235S, pH 6.4, 40°C Aspergillus niger
1.05
-
 methyl ferulate mutant D93G/S187F, pH 6.4, 40°C Aspergillus niger
1.21
-
 methyl ferulate mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R, pH 6.4, 40°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 assay at Aspergillus niger

General Information

General Information Comment Organism
additional information homology-based modelling, overview Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.14
-
 methyl ferulate mutant C235S, pH 6.4, 40°C Aspergillus niger
0.195
-
 methyl ferulate mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R/C235S, pH 6.4, 40°C Aspergillus niger
0.29
-
 methyl ferulate mutant D93G/S187F, pH 6.4, 40°C Aspergillus niger
0.305
-
 methyl ferulate mutant L14F/T35I/K37I/T57I/T63I/A140T/Q121H/S163T/Q177H/V178A/Q185R, pH 6.4, 40°C Aspergillus niger