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Literature summary for 3.1.1.73 extracted from

  • Esteban-Torres, M.; Reveron, I.; Mancheno, J.M.; de Las Rivas, B.; Munoz, R.
    Characterization of a feruloyl esterase from Lactobacillus plantarum (2013), Appl. Environ. Microbiol., 79, 5130-5136.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.73

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100 Lactiplantibacillus plantarum
Tween 20 250% activation at 1 mM Lactiplantibacillus plantarum
Tween 80 250% activation at 1 mM Lactiplantibacillus plantarum
ZnCl2 155% activation at 1 mM Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
gene lp_0796, cloning in Escherichia coli strain DH10B, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) from plasmid pURI3-TEV-0796 Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Lactiplantibacillus plantarum
CuCl2
-
 Lactiplantibacillus plantarum
diethyldicarbonate
-
 Lactiplantibacillus plantarum
DTT strong inhibition Lactiplantibacillus plantarum
EDTA
-
 Lactiplantibacillus plantarum
ethyl acetate
-
 Lactiplantibacillus plantarum
HgCl2 strong inhibition Lactiplantibacillus plantarum
KCl
-
 Lactiplantibacillus plantarum
additional information the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100 Lactiplantibacillus plantarum
PMSF strong inhibition Lactiplantibacillus plantarum
pyridoxal 5'-phosphate
-
 Lactiplantibacillus plantarum
SDS strong inhibition Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100 Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum F9UM18 gene lp_0796
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl cinnamate + H2O
-
 Lactiplantibacillus plantarum benzyl alcohol + cinnamate
-
 ?
butyl acetate + H2O
-
 Lactiplantibacillus plantarum butanol + acetate
-
 ?
ethyl acetate + H2O
-
 Lactiplantibacillus plantarum ethanol + acetate
-
 ?
ethyl bromoacetate + H2O
-
 Lactiplantibacillus plantarum ethanol + bromoacetate
-
 ?
ethyl butanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + butyrate
-
 ?
ethyl decanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + decanoate
-
 ?
ethyl glycine + H2O
-
 Lactiplantibacillus plantarum ethanol + glycine
-
 ?
ethyl hexanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + hexanoate
-
 ?
ethyl octanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + octanoate
-
 ?
ethyl oleate + H2O
-
 Lactiplantibacillus plantarum ethanol + oleate
-
 ?
isobutyl acetate + H2O
-
 Lactiplantibacillus plantarum isobutanol + acetate
-
 ?
isopropenyl acetate + H2O
-
 Lactiplantibacillus plantarum isopropenol + acetate
-
 ?
methyl (4-hydroxymethyl)benzoate + H2O
-
 Lactiplantibacillus plantarum methanol + (4-hydroxymethyl)benzoate
-
 ?
methyl 4-coumarate + H2O
-
 Lactiplantibacillus plantarum 4-coumarate + methanol
-
 ?
methyl benzoate + H2O
-
 Lactiplantibacillus plantarum methanol + benzoate
-
 ?
methyl bromoacetate + H2O
-
 Lactiplantibacillus plantarum methanol + bromoacetate
-
 ?
methyl caffeate + H2O
-
 Lactiplantibacillus plantarum caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Lactiplantibacillus plantarum ferulate + methanol
-
 ?
methyl hydroxyacetate + H2O
-
 Lactiplantibacillus plantarum methanol + hydroxyacetate
-
 ?
methyl mandelate + H2O
-
 Lactiplantibacillus plantarum methanol + mandelate
-
 ?
methyl phenylacetate + H2O
-
 Lactiplantibacillus plantarum methanol + phenylacetate
-
 ?
methyl sinapinate + H2O
-
 Lactiplantibacillus plantarum sinapate + methanol
-
 ?
additional information the enzyme Lp_0796 is an esterase that, among other substrates, is able to efficiently hydrolyze the four model substrates for feruloyl esterases. No or poor activity with vinyl octanoate, ethyl trifluoroacetate, rosmarinic acid, chlorogenic acid, and trilaurin Lactiplantibacillus plantarum ?
-
 ?
phenyl acetate + H2O
-
 Lactiplantibacillus plantarum phenol + acetate
-
 ?
propyl acetate + H2O
-
 Lactiplantibacillus plantarum propanol + acetate
-
 ?
triacetin + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
tributyrin + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
vinyl acetate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + acetate
-
 ?
vinyl benzoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + benzoate
-
 ?
vinyl butanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + butanoate
-
 ?
vinyl decanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + decanoate
-
 ?
vinyl hexadecanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + hexadecanoate
-
 ?
vinyl hexanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + hexanoate
-
 ?
vinyl propanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + propanoate
-
 ?
vinyl tetradecanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + tetradecanoate
-
 ?
vinyl trimethylacetate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + trimethylacetate
-
 ?

Synonyms

Synonyms Comment Organism
Lp_0796
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Lactiplantibacillus plantarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 45 marginal activity at 45°C Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Lactiplantibacillus plantarum

Expression

Organism Comment Expression
Lactiplantibacillus plantarum the enzyme is induced by 3 mM methyl ferulate up