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Literature summary for 3.1.1.73 extracted from

  • Wang, L.; Zhang, R.; Ma, Z.; Wang, H.; Ng, T.
    A feruloyl esterase (FAE) characterized by relatively high thermostability from the edible mushroom Russula virescens (2014), Appl. Biochem. Biotechnol., 172, 993-1003.
    View publication on PubMed
Search for more literature for 3.1.1.73

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 23% inhibition at 2.5 mM, 45% inhibition at 5 mM Russula virescens
Cd2+ 31% inhibition at 5 mM Russula virescens
Cu2+ 20% inhibition at 5 mM, 37% at 10 mM Russula virescens
Fe2+ complete inhibition at 5 mM Russula virescens
Hg2+ 60% inhibition at 1.25 mM, about 70% inhibition at 2.5-10 mM Russula virescens
K+ 10% inhibition at 5 mM, 20% at 10 mM Russula virescens
Mg2+ 8.5% inhibition at 5 mM, 22% at 10 mM Russula virescens
Pb2+ complete inhibition at 2.5 mM Russula virescens
Zn2+ 47% inhibition at 10 mM Russula virescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 methyl ferulate pH 5.0, 50°C Russula virescens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme activity is not significantly affected by Mn2+ at 2.5 mM Russula virescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 gel filtration Russula virescens

Organism

Organism UniProt Comment Textmining
Russula virescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 29.2fold from fruiting bodies by several steps of cation and anion exchange chromatography, and gel filtration Russula virescens

Source Tissue

Source Tissue Comment Organism Textmining
fruit body fresh Russula virescens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butanoate + H2O
-
 Russula virescens 4-nitrophenol + butanoate
-
 ?
methyl 4-coumarate + H2O
-
 Russula virescens 4-coumarate + methanol
-
 ?
methyl caffeate + H2O
-
 Russula virescens caffeic acid + methanol
-
 ?
methyl ferulate + H2O preferred substrate Russula virescens ferulate + methanol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62000 Russula virescens

Synonyms

Synonyms Comment Organism
FAE
-
 Russula virescens
ferulic acid esterase
-
 Russula virescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Russula virescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 activity range, overview Russula virescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 purified native enzyme, 60 min, stable Russula virescens
70
-
 purified native enzyme, 60 min, over 70% residual activity Russula virescens
80
-
 purified native enzyme, 60 min, over 34% residual activity Russula virescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Russula virescens

pH Range

pH Minimum pH Maximum Comment Organism
4 6 75% of maximal activity at pH 4.0, maximal activity at pH 5.0, and 67% of maximal activity at pH 6.0, enzyme activity declined sharply above Russula virescens

General Information

General Information Comment Organism
evolution feruloyl esterases are a subclass of the carboxylic acid esterases, E.C. 3.1.1.1, that are able to hydrolyze the ester bond between hydroxycinnamic acids and sugars present in the plant cell walls Russula virescens