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Literature summary for 3.1.1.73 extracted from
- Characterization of two distinct feruloyl esterases, AoFaeB and AoFaeC, from Aspergillus oryzae (2009), Appl. Microbiol. Biotechnol., 83, 689-696.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | ferulic acid release by recombinant FaeB or FaeC is increased considerably by 17.6fold and 16fold, respectively in the presence of Thermomyces lanuginosus xylanase | Aspergillus oryzae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PCR product cloned into the pGEM-T Easy vector. FaeB and FaeC fragments subcloned into EcoRI-XbaI-digested pPICZalphaA expression vector. FaeB and FaeC expressed in Pichia pastoris GS115 by fusing to the Saccharomyces cerevisiae alpha-factor secretion signal peptide | Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
| 61000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
| 75000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Aspergillus oryzae RIB 40 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| FaeB and FaeC, in a two-step procedure using anion-exchange chromatography and gel filtration | Aspergillus oryzae |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAEB | - |
Aspergillus oryzae |
| FaeC | - |
Aspergillus oryzae |
| feruloyl esterase | - |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purified recombinant FaeB and FaeC retain more than 90% of their activity after incubation at 55°C for 30 min. FaeC retains 40% of its activity after treatment at 65°C, whereas FaeB almost loses its activity | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
purified recombinant FaeB and FaeC | Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | purified recombinant FaeB and FaeC show more than 90% of their maximum activity | Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | purified recombinant FaeB | Aspergillus oryzae |
| 7 | 10 | purified recombinant FaeC | Aspergillus oryzae |
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