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Literature summary for 3.1.1.73 extracted from
- Purification and characterization of ferulic acid esterase from malted finger millet (Eleusine coracana, Indaf-15) (2007), J. Agric. Food Chem., 55, 9704-9712.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| oxalic acid | 5 mM, 1.4fold activation | Eleusine coracana |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 5 mM, complete inactivation | Eleusine coracana | |
| iodoacetamide | 50 mM, 45% inhibition | Eleusine coracana | |
| p-chloromercuric benzoate | 50 mM, 67% inhibition | Eleusine coracana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000053 | - |
p-nitrophenylferulate | - |
Eleusine coracana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, 1.4fold activation | Eleusine coracana | |
| Cu+ | 5 mM, 2fold activation | Eleusine coracana | |
| Cu2+ | 5 mM, 2.17fold activation | Eleusine coracana | |
| Ni2+ | 5 mM, 1.4fold activation | Eleusine coracana | |
| Zn2+ | 5 mM, 1.4fold activation | Eleusine coracana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16500 | - |
gel filtration | Eleusine coracana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eleusine coracana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Eleusine coracana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.8 | - |
- |
Eleusine coracana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | ferulic acid is enzymatically released from water-soluble polysaccharides and synthetic substrates | Eleusine coracana | ? | - |
? | |
| p-nitrophenylferulate + H2O | - |
Eleusine coracana | ferulate + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 16500, SDS-PAGE | Eleusine coracana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Eleusine coracana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 60% of maximal activity, 60°C: about 65% of maximal activity | Eleusine coracana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
15 min, stable | Eleusine coracana |
| 40 | - |
15 min, 20% loss of activity | Eleusine coracana |
| 50 | - |
15 min, about 70% of maximal activity | Eleusine coracana |
| 60 | - |
15 min, 50% loss of activity | Eleusine coracana |
| 70 | - |
15 min, 80% loss of activity | Eleusine coracana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Eleusine coracana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | pH 5.5: about 70% of maximal activity, pH 8.0: about 50% of maximal activity | Eleusine coracana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | 2 h, 10-20% loss of activity | Eleusine coracana |
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