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Literature summary for 3.1.1.73 extracted from
- A type B feruloyl esterase from Aspergillus nidulans with broad pH applicability (2007), Appl. Microbiol. Biotechnol., 73, 1323-1330.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| successfully cloned from the genomic DNA and expressed in Saccharomyces cerevisiae | Aspergillus nidulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.248 | - |
methyl ferulate | 40°C, pH 7.0 | Aspergillus nidulans |  |
| 0.387 | - |
methyl caffeate | 40°C, pH 7.0 | Aspergillus nidulans | |
| 0.659 | - |
methyl p-coumarate | 40°C, pH 7.0 | Aspergillus nidulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, deglycosylated enzyme, SDS-PAGE | Aspergillus nidulans |
| 56107 | - |
x * 56107, calculated from sequence | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.9 | - |
- |
Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl caffeate + H2O | - |
Aspergillus nidulans | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Aspergillus nidulans | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + H2O | - |
Aspergillus nidulans | p-coumaric acid + methanol | - |
? | |
| additional information | no activity with methyl sinapate | Aspergillus nidulans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, deglycosylated enzyme, SDS-PAGE | Aspergillus nidulans |
| ? | x * 56107, calculated from sequence | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AN1772.2 | - |
Aspergillus nidulans |
| type B FAE | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Aspergillus nidulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 80% of maximal activity, 50°C: about 30% of maximal activity | Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable up to | Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aspergillus nidulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5.0: about 50% of maximal activity, pH 8.5: about 40% of maximal activity | Aspergillus nidulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
4°C, 12 h, 15% loss of activity | Aspergillus nidulans |
| 8 | - |
4°C, 12 h, no loss of activity | Aspergillus nidulans |
| 10 | - |
4°C, 12 h, about 20% loss of activity | Aspergillus nidulans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus nidulans | calculated from sequence | - |
4.7 |
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