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Literature summary for 3.1.1.73 extracted from
- The feruloyl esterase system of Talaromyces stipitatus: Determining the hydrolytic and synthetic specificity of TsFaeC (2006), J. Biotechnol., 125, 210-221.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential use for providing ferulic acid as precursor for the synthesis of cinnamic acid and its derivatives as anti-cancer drugs and for antimicrobial, antiviral and anti-inflammatory agents | Talaromyces stipitatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Talaromyces stipitatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
methyl 4-hydroxy cinnamate | - |
Talaromyces stipitatus |  |
| 0.015 | - |
methyl 2-hydroxy cinnamate | - |
Talaromyces stipitatus | |
| 0.016 | - |
methyl 4-hydroxy-3-methoxy phenyl propionate | - |
Talaromyces stipitatus | |
| 0.025 | - |
methyl 3-hydroxy cinnamate | - |
Talaromyces stipitatus | |
| 0.035 | - |
iso-propyl ferulate | - |
Talaromyces stipitatus | |
| 0.035 | - |
iso-propyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 0.04 | - |
methyl ferulate | - |
Talaromyces stipitatus | |
| 0.04 | - |
n-propyl ferulate | - |
Talaromyces stipitatus | |
| 0.04 | - |
methyl 4-hydroxy-3-methoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.04 | - |
methyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 0.04 | - |
n-propyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 0.045 | - |
ethyl ferulate | - |
Talaromyces stipitatus | |
| 0.045 | - |
ethyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 0.05 | - |
2-butyl ferulate | - |
Talaromyces stipitatus | |
| 0.05 | - |
2-butyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 0.075 | - |
methyl 3,5-dimethoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.076 | - |
iso-butyl ferulate | - |
Talaromyces stipitatus | |
| 0.076 | - |
iso-butyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 0.106 | - |
n-butyl ferulate | - |
Talaromyces stipitatus | |
| 0.106 | - |
n-butyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 0.118 | - |
methyl cinnamate | - |
Talaromyces stipitatus | |
| 0.14 | - |
methyl 3,4-dihydroxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.22 | - |
methyl 3-hydroxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.301 | - |
methyl 3-methoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.33 | - |
methyl cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.37 | - |
methyl 4-hydroxy-3,5-dimethoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.47 | - |
methyl 3-methoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.533 | - |
methyl 3-hydroxy-4-methoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.57 | - |
methyl 4-hydroxy-3,5-dimethoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.59 | - |
methyl 4-hydroxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.626 | - |
methyl 3,4-dimethoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.63 | - |
methyl 3-hydroxy-4-methoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.65 | - |
methyl 3,4-dimethoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.76 | - |
methyl 3,4-dihydroxy-phenyl propionate | - |
Thermothelomyces heterothallicus | |
| 1.06 | - |
methyl 4-hydroxy-3-methoxy phenyl propionate | - |
Thermothelomyces heterothallicus | |
| 1.64 | - |
methyl 4-hydroxy-3-methoxy cinnamate | - |
Thermothelomyces heterothallicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Talaromyces stipitatus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | Talaromyces stipitatus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + diferulic acid + ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| n-hexane | n-hexane/t-butanol/water, suitable solvent for transesterification | Talaromyces stipitatus |
| t-butanol | n-hexane/t-butanol/water, suitable solvent for transesterification | Talaromyces stipitatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces stipitatus | - |
- |
- |
| Talaromyces stipitatus | - |
expressed in Pichia pastoris | - |
| Thermothelomyces heterothallicus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-butyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + 2-butanol | - |
? | |
| 3-[3-(3,4 dihydroxyphenyl)acryloyl] oxy-1,4,5-trihydroxycyclohexane-1-carboxylic acid + H2O | - |
Thermothelomyces heterothallicus | ? | - |
? | |
| 3-[3-(3-4 dihydroxyphenyl)acryloyl] oxy-1,4,5 trihydroxycyclohexane-1-carboxylic acid + H2O | - |
Talaromyces stipitatus | ? | - |
? | |
| ethyl cinnamate + H2O | - |
Talaromyces stipitatus | cinnamic acid + ethanol | - |
? | |
| ethyl cinnamate + H2O | - |
Thermothelomyces heterothallicus | cinnamic acid + ethanol | - |
? | |
| ethyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + ethanol | - |
? | |
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Talaromyces stipitatus | ferulic acid + diferulic acid + ? | - |
? | |
| iso-butyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + iso-butanol | - |
? | |
| iso-propyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + iso-propanol | - |
? | |
| methyl 2-hydroxycinnamate + H2O | - |
Talaromyces stipitatus | 2-hydroxycinnamic acid + methanol | - |
? | |
| methyl 3,4-dihydroxy-phenyl propionate + H2O | - |
Talaromyces stipitatus | methanol + 3,4-dihydroxy-phenyl propionic acid | - |
? | |
| methyl 3,4-dihydroxy-phenyl propionate + H2O | - |
Thermothelomyces heterothallicus | methanol + 3,4-dihydroxy-phenyl propionic acid | - |
? | |
| methyl 3,4-dihydroxycinnamate + H2O | - |
Talaromyces stipitatus | 3,4-dihydroxycinnamic acid + methanol | - |
? | |
| methyl 3,4-dihydroxycinnamate + H2O | - |
Thermothelomyces heterothallicus | 3,4-dihydroxycinnamic acid + methanol | - |
? | |
| methyl 3,4-dimethoxy cinnamate + H2O | - |
Talaromyces stipitatus | 3,4-dimethoxy cinnamic acid + methanol | - |
? | |
| methyl 3,4-dimethoxy cinnamate + H2O | - |
Thermothelomyces heterothallicus | 3,4-dimethoxy cinnamic acid + methanol | - |
? | |
| methyl 3,4-methylene dioxy phenyl propionate + H2O | - |
Talaromyces stipitatus | methanol + 3,4-methylene dioxy phenyl propionic acid | - |
? | |
| methyl 3,4-methylene dioxyphenyl propionate + H2O | - |
Thermothelomyces heterothallicus | methyl 3,4-methylene dioxyphenyl propionic acid + methanol | - |
? | |
| methyl 3,5-dimethoxy cinnamate + H2O | - |
Talaromyces stipitatus | 3,5-dimethoxy cinnamic acid + methanol | - |
? | |
| methyl 3-hydroxy-4-methoxycinnamate + H2O | - |
Thermothelomyces heterothallicus | methanol + 3-hydroxy-4-methoxycinnamic acid | - |
? | |
| methyl 3-hydroxy-4-methoxycinnamate + H2O | - |
Talaromyces stipitatus | 3-hydroxy-4-methoxycinnamic acid + methanol | - |
? | |
| methyl 3-hydroxycinnamate + H2O | - |
Talaromyces stipitatus | 3-hydroxycinnamic acid + methanol | - |
? | |
| methyl 3-hydroxycinnamate + H2O | - |
Thermothelomyces heterothallicus | 3-hydroxycinnamic acid + methanol | - |
? | |
| methyl 3-methoxycinnamate + H2O | - |
Thermothelomyces heterothallicus | methanol + 3-methoxycinnamic acid | - |
? | |
| methyl 3-methoxycinnamate + H2O | - |
Talaromyces stipitatus | 3-methoxycinnamic acid + methanol | - |
? | |
| methyl 4-hydroxy-3,5-dimethoxycinnamate + H2O | - |
Talaromyces stipitatus | 4-hydroxy-3,5-dimethoxycinnamic acid + methanol | - |
? | |
| methyl 4-hydroxy-3-methoxycinnamate + H2O | - |
Talaromyces stipitatus | 4-hydroxy-3-methoxycinnamic acid + methanol | - |
? | |
| methyl 4-hydroxy-3-methoxycinnamate + H2O | - |
Thermothelomyces heterothallicus | 4-hydroxy-3-methoxycinnamic acid + methanol | - |
? | |
| methyl 4-hydroxy-3-methoxyphenylpropionate + H2O | - |
Talaromyces stipitatus | 4-hydroxy-3-methoxyphenylpropionic acid + methanol | - |
? | |
| methyl 4-hydroxy-3-methoxyphenylpropionate + H2O | - |
Thermothelomyces heterothallicus | 4-hydroxy-3-methoxyphenylpropionic acid + methanol | - |
? | |
| methyl 4-hydroxycinnamate + H2O | - |
Talaromyces stipitatus | 4-hydroxycinnamic acid + methanol | - |
? | |
| methyl 4-hydroxycinnamate + H2O | - |
Thermothelomyces heterothallicus | 4-hydroxycinnamic acid + methanol | - |
? | |
| methyl cinnamate + H2O | - |
Talaromyces stipitatus | cinnamic acid + methanol | - |
? | |
| methyl cinnamate + H2O | - |
Thermothelomyces heterothallicus | cinnamic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + methanol | - |
? | |
| methyl ferulate + H2O | preferred substrate | Talaromyces stipitatus | ferulic acid + methanol | - |
? | |
| methyl ferulate + L-arabinose | 20% transesterification | Talaromyces stipitatus | L-arabinosyl ferulate + methanol | - |
? | |
| methyl ferulate + L-arabinose | transesterification | Talaromyces stipitatus | ? | - |
? | |
| methyl ferulate + L-arabinose | transesterification | Thermothelomyces heterothallicus | ? | - |
? | |
| additional information | numerous derivatives of cinnamic acid esters tested for activity | Talaromyces stipitatus | ? | - |
? | |
| additional information | the other ferulic acid esters tested for hydrolysis show transesterification at rates between 0.2 and 1.4% | Talaromyces stipitatus | ? | - |
? | |
| n-butyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + n-butanol | - |
? | |
| n-butyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + butanol | - |
? | |
| n-propyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + n-propanol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| StFaeC | - |
Thermothelomyces heterothallicus |
| TsFaeC | - |
Talaromyces stipitatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Talaromyces stipitatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
methyl cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.05 | - |
methyl 3-hydroxy-4-methoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.067 | - |
methyl 3,4-dimethoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.1 | - |
methyl 3,5-dimethoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.117 | - |
methyl 3-hydroxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.133 | - |
methyl 3,4-dihydroxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.183 | - |
methyl 3,4-dihydroxy-phenyl propionate | - |
Thermothelomyces heterothallicus | |
| 0.22 | - |
methyl 3-methoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.25 | - |
methyl 3,4-dimethoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.4 | - |
methyl 4-hydroxy-3,5-dimethoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.533 | - |
methyl 4-hydroxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 0.7 | - |
methyl 3-hydroxy-4-methoxy cinnamate | - |
Talaromyces stipitatus | |
| 0.87 | - |
methyl 4-hydroxy-3-methoxy phenyl propionate | - |
Thermothelomyces heterothallicus | |
| 2.2 | - |
methyl 2-hydroxy cinnamate | - |
Talaromyces stipitatus | |
| 2.6 | - |
methyl 4-hydroxy-3-methoxy cinnamate | - |
Thermothelomyces heterothallicus | |
| 3.5 | - |
methyl cinnamate | - |
Talaromyces stipitatus | |
| 9.3 | - |
methyl 4-hydroxy-3,5-dimethoxy cinnamate | - |
Talaromyces stipitatus | |
| 9.55 | - |
methyl 4-hydroxy cinnamate | - |
Talaromyces stipitatus | |
| 9.65 | - |
methyl ferulate | - |
Talaromyces stipitatus | |
| 9.65 | - |
methyl 4-hydroxy-3-methoxy cinnamate | - |
Talaromyces stipitatus | |
| 10 | - |
methyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 15.9 | - |
methyl 3-methoxy cinnamate | - |
Talaromyces stipitatus | |
| 20.27 | - |
methyl cinnamate | - |
Talaromyces stipitatus | |
| 24.1 | - |
methyl 3-hydroxy cinnamate | - |
Talaromyces stipitatus | |
| 27.28 | - |
methyl 3-methoxy cinnamate | - |
Talaromyces stipitatus | |
| 64.45 | - |
methyl 3,4-dihydroxy-phenyl propionate | - |
Talaromyces stipitatus | |
| 125.9 | - |
methyl 4-hydroxy-3-methoxy phenyl propionate | - |
Talaromyces stipitatus | |
| 142 | - |
methyl 3,4-dihydroxy-phenyl propionate | - |
Talaromyces stipitatus | |
| 1345 | - |
2-butyl ferulate | - |
Talaromyces stipitatus | |
| 1345 | - |
2-butyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 1358 | - |
n-propyl ferulate | - |
Talaromyces stipitatus | |
| 1358 | - |
n-propyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 2644 | - |
iso-propyl ferulate | - |
Talaromyces stipitatus | |
| 2645 | - |
iso-propyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 2989 | - |
iso-butyl ferulate | - |
Talaromyces stipitatus | |
| 2989 | - |
iso-butyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 3378 | - |
n-butyl ferulate | - |
Talaromyces stipitatus | |
| 3378 | - |
n-butyl ferulate | hydrolysis | Talaromyces stipitatus | |
| 9570 | - |
ethyl ferulate | - |
Talaromyces stipitatus | |
| 9570 | - |
ethyl ferulate | hydrolysis | Talaromyces stipitatus | |
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