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Literature summary for 3.1.1.73 extracted from

  • Koseki, T.; Takahashi, K.; Fushinobu, S.; Iefuji, H.; Iwano, K.; Hashizume, K.; Matsuzawa, H.
    Mutational analysis of a feruloyl esterase from Aspergillus awamori involved in substrate discrimination and pH dependence (2005), Biochim. Biophys. Acta, 1722, 200-208.
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aspergillus awamori

Protein Variants

Protein Variants Comment Organism
D77I higher catalytic efficiency towards alpha-naphtylbutyrate and alpha-naphtylcaprylate, some activity towards long-acyl chain esters, no activity towards phenolic acid methyl esters and feruloylated arabinoxylan, increased pH-optimum Aspergillus awamori
D77N increased pH-optimum Aspergillus awamori
L74W no enzyme protein detected Aspergillus awamori
S172A inactive Aspergillus awamori
T72R minor effect on activity Aspergillus awamori
Y80F higher catalytic efficiency towards alpha-naphtylbutyrate and alpha-naphtylcaprylate, increased pH-optimum Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 alpha-naphthylcaprylate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
0.02
-
 alpha-naphthylcaprylate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori
0.05
-
 alpha-naphthylcaprylate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
0.079
-
 alpha-naphthylcaprylate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
0.15
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77I mutant enzyme Aspergillus awamori
0.18
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77N mutant enzyme Aspergillus awamori
0.29
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
0.33
-
 alpha-naphthylbutyrate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
0.63
-
 alpha-naphthylbutyrate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
0.63
-
 alpha-naphthylbutyrate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus awamori
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Aspergillus awamori involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O Aspergillus awamori IFO 4033 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus awamori Q9P979 feruloyl esterase type A
-
Aspergillus awamori IFO 4033 Q9P979 feruloyl esterase type A
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl butyrate + H2O
-
 Aspergillus awamori 1-naphthol + butanoate
-
 ?
1-naphthyl butyrate + H2O
-
 Aspergillus awamori IFO 4033 1-naphthol + butanoate
-
 ?
1-naphthyl caprylate + H2O
-
 Aspergillus awamori caprylic acid + 1-naphthol
-
 ?
feruloyl arabinoxylan + H2O
-
 Aspergillus awamori ferulic acid + arabinoxylan
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus awamori ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus awamori IFO 4033 ferulic acid + ?
-
 ?
methyl caffeate + H2O
-
 Aspergillus awamori caffeic acid + methanol
-
 ?
methyl caffeate + H2O
-
 Aspergillus awamori IFO 4033 caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Aspergillus awamori ferulic acid + methanol
-
 ?
methyl p-coumarate + H2O
-
 Aspergillus awamori p-coumaric acid + methanol
-
 ?
methyl sinapinate + H2O
-
 Aspergillus awamori sinapic acid + methanol
-
 ?
methyl sinapinate + H2O
-
 Aspergillus awamori IFO 4033 sinapic acid + methanol
-
 ?
additional information active on alpha-naphthyl esters with chain length from C2 to C8, highest activity towards C4 ester Aspergillus awamori ?
-
 ?
additional information active on alpha-naphthyl esters with chain length from C2 to C8, highest activity towards C4 ester Aspergillus awamori IFO 4033 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
 alpha-naphthylcaprylate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
0.64
-
 alpha-naphthylcaprylate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
0.85
-
 alpha-naphthylcaprylate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
1.09
-
 alpha-naphthylcaprylate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori
2.33
-
 alpha-naphthylbutyrate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
2.7
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
3.38
-
 alpha-naphthylbutyrate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori
3.72
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77I mutant enzyme Aspergillus awamori
3.77
-
 alpha-naphthylbutyrate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
32.1
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77N mutant enzyme Aspergillus awamori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 wild type, D71I, and T72R mutant enzymes Aspergillus awamori
6
-
 Y80F mutant enzyme Aspergillus awamori
7 8 D77I mutant enzyme Aspergillus awamori
7
-
 D77N mutant enzyme Aspergillus awamori