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Literature summary for 3.1.1.73 extracted from

  • De Vries, R.P.; Michelsen, B.; Poulsen, C.H.; Kroon, P.A.; van den Heuvel, R.H.; Faulds, C.B.; Williamson, G.; van den Hombergh, J.P.; Visser, J.
    The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides (1997), Appl. Environ. Microbiol., 63, 4638-4644.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene cinI, coding for cinnamoyl esterase Butyrivibrio fibrisolvens
PCR cloning and sequence analysis, gene faeA encoding ferulic acid esterase cloned, the gene product FAEA overexpressed in wild-type Aspergillus tubingensis and protease-deficient Aspergillus niger mutant, gene product is identical with Aspergillus niger FAE-III Aspergillus niger
PCR cloning and sequence analysis, gene faeA encoding ferulic acid esterase cloned, the gene product FAEA overexpressed in wild-type Aspergillus tubingensis and protease-deficient Aspergillus niger mutant, gene product is identical with Aspergillus niger FAE-III Aspergillus tubingensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.72
-
methyl ferulate FAE-III Aspergillus niger
0.76
-
methyl ferulate recombinant FAEA Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29740
-
FAEA, mass spectrometry Aspergillus tubingensis
29740
-
FAE-III, mass spectrometry Aspergillus niger
36000
-
-
Aspergillus tubingensis
36000
-
FAE-III Aspergillus niger
36000
-
FAEA Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arabinoxylans + H2O Aspergillus niger
-
ferulic acid + ?
-
?
arabinoxylans + H2O Aspergillus tubingensis
-
ferulic acid + ?
-
?
arabinoxylans + H2O Aspergillus niger N402
-
ferulic acid + ?
-
?
feruloyl-polysaccharide + H2O Aspergillus niger
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Butyrivibrio fibrisolvens
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Aspergillus tubingensis
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Aspergillus niger N402
-
ferulate + polysaccharide
-
?
sugar beet pectin + H2O Aspergillus tubingensis
-
ferulic acid + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
and strain NW219
-
Aspergillus niger N402
-
and strain NW219
-
Aspergillus tubingensis
-
-
-
Butyrivibrio fibrisolvens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger
recombinant gene product Aspergillus tubingensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylans + H2O
-
Aspergillus niger ferulic acid + ?
-
?
arabinoxylans + H2O
-
Aspergillus tubingensis ferulic acid + ?
-
?
arabinoxylans + H2O
-
Aspergillus niger N402 ferulic acid + ?
-
?
ferulic acid methyl ester + H2O
-
Aspergillus niger ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Aspergillus tubingensis ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Aspergillus niger N402 ferulic acid + methanol
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus niger ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Butyrivibrio fibrisolvens ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus tubingensis ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus niger N402 ferulate + polysaccharide
-
?
sugar beet pectin + H2O
-
Aspergillus tubingensis ferulic acid + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60 FAE-III Aspergillus niger
60
-
-
Aspergillus niger
60
-
-
Aspergillus tubingensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus niger
5
-
-
Aspergillus tubingensis