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Literature summary for 3.1.1.72 extracted from
- Heterologous expression and biochemical characterization of acetyl xylan esterase from Coprinopsis cinerea (2013), World J. Microbiol. Biotechnol., 29, 597-605.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene axe, functional expression of the enzyme with a C-terminal His-tag under the alcohol oxidase 1 promoter in Pichia pastoris strain KM71, the recombinant enzyme is secreted, subcloning in Escherichia coli strain TOP10 | Coprinopsis cinerea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | presence of two additional amino acid residues at the enzyme's native N-terminus help stabilize the enzyme against the protease cleavages without affecting the enzyme activity | Coprinopsis cinerea |
General Stability
| General Stability | Organism |
|---|---|
| presence of two additional amino acid residues at the enzyme's native N-terminus help stabilize the enzyme against the protease cleavages without affecting the enzyme activity | Coprinopsis cinerea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
4-nitrophenyl butyrate | pH 8.0, 40°C, recombinant enzyme | Coprinopsis cinerea |  |
| 4.3 | - |
4-nitrophenyl acetate | pH 8.0, 40°C, recombinant enzyme | Coprinopsis cinerea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
| 39966 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
| 40500 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
| 65500 | - |
x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylated xylan + H2O | Coprinopsis cinerea | - |
partially deacetylated xylan + acetate | - |
? | |
| acetylated xylan + H2O | Coprinopsis cinerea Okayama 7 | - |
partially deacetylated xylan + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | Q09IZ4 | - |
- |
| Coprinopsis cinerea Okayama 7 | Q09IZ4 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme has two potential N-glycosylation site, presence of N-glycosylation on the recombinant enzyme with predominant N-glycans HexNAc2Hex9-16, deglycosylation by PNGase F | Coprinopsis cinerea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminal His-tagged enzyme from Pichia pastoris strain KM71 by nickel affinity chromatography | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Coprinopsis cinerea | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Coprinopsis cinerea Okayama 7 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Coprinopsis cinerea | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Coprinopsis cinerea Okayama 7 | 4-nitrophenol + butyrate | - |
? | |
| acetylated xylan + H2O | - |
Coprinopsis cinerea | partially deacetylated xylan + acetate | - |
? | |
| acetylated xylan + H2O | - |
Coprinopsis cinerea Okayama 7 | partially deacetylated xylan + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65500, recombinant enzyme, SDS-PAGE, x * 39966, sequence calculation, x * 35000, recombinant His-tagged and deglycosylated enzyme, SDS-PAGE, x * 40500, about, unglycosylated His-tagged enzyme, sequence calculation | Coprinopsis cinerea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl xylan esterase | - |
Coprinopsis cinerea |
| AXE | - |
Coprinopsis cinerea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme, sharp drop in activity above the optimum at 40°C | Coprinopsis cinerea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 45 | activity range, profile overview | Coprinopsis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Coprinopsis cinerea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | activity range, profile overview | Coprinopsis cinerea |
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