Literature summary for 3.1.1.72 extracted from

Lansky, S.; Alalouf, O.; Salama, R.; Dvir, H.; Shoham, Y.; Shoham, G.
Preliminary crystallographic analysis of a double mutant of the acetyl xylo-oligosaccharide esterase Axe2 in its dimeric form (2014), Acta Crystallogr. Sect. F, 70, 476-481.

Cloned(Commentary)

Cloned (Comment)	Organism
gene axe2, overexpression of mutant Y184F/W190P in Escherichia coli strain BL21(DE3)	Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme mutant Y184F/W190P, hanging drop vapour diffusion method, mixing of 0.0025 ml of 3 mg/ml protein in 50 mM TrisHCl pH 7.0, 100 mM NaCl, and 0.02% NaN3, with 0.0025 ml of reservoir solution containing 16-21% PEG 3000, 0.1 M Tris-HCl, pH 7.5, and 200 mM calcium acetate, and equilibration over 0.5-1.0 ml reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.3 A resolution	Geobacillus stearothermophilus

Crystallization (Comment)

Organism

purified recombinant enzyme mutant Y184F/W190P, hanging drop vapour diffusion method, mixing of 0.0025 ml of 3 mg/ml protein in 50 mM TrisHCl pH 7.0, 100 mM NaCl, and 0.02% NaN3, with 0.0025 ml of reservoir solution containing 16-21% PEG 3000, 0.1 M Tris-HCl, pH 7.5, and 200 mM calcium acetate, and equilibration over 0.5-1.0 ml reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.3 A resolution

Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
Y184F/W190P	site-directed mutagenesis, a dimeric enzyme mutant that shows a significant reduction in catalytic activity compared to the wild-type enzyme, structure comparison with the wild-type, overview	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q09LX1	gene axe2	-
Geobacillus stearothermophilus T-6	Q09LX1	gene axe2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant mutant Y184F/W190P from Escherichia coli strain BL21(DE3) by protamine precipitation and gel filtration	Geobacillus stearothermophilus

Subunits

Subunits	Comment	Organism
octamer	the wild-type enzyme is present as a unique torus-shaped octamer in the crystal and in solution	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
acetyl xylo-oligosaccharide esterase	-	Geobacillus stearothermophilus
Axe2	-	Geobacillus stearothermophilus