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Literature summary for 3.1.1.72 extracted from
- Purification and properties of an acetylxylan esterase from Thermobifida fusca (2008), Enzyme Microb. Technol., 42, 181-186.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli DH5 alpha | Thermobifida fusca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | - |
Thermobifida fusca |  |
| Hg2+ | - |
Thermobifida fusca | |
| phenylmethanesulfonyl fluoride | - |
Thermobifida fusca | |
| Zn2+ | - |
Thermobifida fusca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00186 | - |
p-nitrophenyl acetate | 30°C, pH 8.0 | Thermobifida fusca | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermobifida fusca | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration on Sepharose CL-6B | Thermobifida fusca |
| 30000 | - |
SDS-PAGE | Thermobifida fusca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | - |
- |
- |
| Thermobifida fusca NTU22 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| centrifugation, ultrafiltration, gel-filtration chromatography, 4°C | Thermobifida fusca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Thermobifida fusca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.6 | - |
purified enzyme | Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylxylan + H2O | - |
Thermobifida fusca | ? | - |
? | |
| acetylxylan + H2O | - |
Thermobifida fusca NTU22 | ? | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Thermobifida fusca | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl butyrate + H2O | - |
Thermobifida fusca | p-nitrophenol + butyrate | - |
? | |
| p-nitrophenyl butyrate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + butyrate | - |
? | |
| p-nitrophenyl caprylate + H2O | - |
Thermobifida fusca | p-nitrophenol + caprylate | - |
? | |
| p-nitrophenyl caprylate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + caprylate | - |
? | |
| p-nitrophenyl palmitate + H2O | - |
Thermobifida fusca | p-nitrophenol + palmitate | - |
? | |
| p-nitrophenyl palmitate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + palmitate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Thermobifida fusca |
| monomer | 1 * 28000, gel filtration on Sepharose CL-6B | Thermobifida fusca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetylxylan esterase | - |
Thermobifida fusca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermobifida fusca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
about 70% of the original activity still remains after 3 h at 70°C | Thermobifida fusca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 47.6 | - |
p-nitrophenyl acetate | 30°C, pH 8.0 | Thermobifida fusca | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermobifida fusca |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Thermobifida fusca |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 4°C, 24 h | Thermobifida fusca |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermobifida fusca | isoelectric focusing gel electrophoresis | - |
6.55 |
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