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Literature summary for 3.1.1.72 extracted from

  • Ding, S.; Cao, J.; Zhou, R.; Zheng, F.
    Molecular cloning, and characterization of a modular acetyl xylan esterase from the edible straw mushroom Volvariella volvacea (2007), FEMS Microbiol. Lett., 274, 304-310.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Volvariella volvacea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3077
-
4-yethylumbelliferyl acetate pH 7.5, 50°C Volvariella volvacea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39900
-
x * 39900, calculated from sequence Volvariella volvacea

Organism

Organism UniProt Comment Textmining
Volvariella volvacea Q09IZ4
-
-
Volvariella volvacea V14 Q09IZ4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Volvariella volvacea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Volvariella volvacea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
Volvariella volvacea 4-methylumbelliferone + acetate
-
?
4-methylumbelliferyl acetate + H2O
-
Volvariella volvacea V14 4-methylumbelliferone + acetate
-
?
acetylxylan + H2O
-
Volvariella volvacea ?
-
?
acetylxylan + H2O
-
Volvariella volvacea V14 ?
-
?
beta-D-xylose tetraacetate + H2O
-
Volvariella volvacea ?
-
?
beta-D-xylose tetraacetate + H2O
-
Volvariella volvacea V14 ?
-
?
additional information alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate Volvariella volvacea ?
-
?
additional information alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate Volvariella volvacea V14 ?
-
?

Subunits

Subunits Comment Organism
? x * 39900, calculated from sequence Volvariella volvacea

Synonyms

Synonyms Comment Organism
Vvaxe1
-
Volvariella volvacea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 50% of maximal activity, 70°C: about 90% of maximal activity, hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 40% of maximal activity, pH 10: about 40% of maximal activity, hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea