Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Volvariella volvacea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3077 | - |
4-yethylumbelliferyl acetate | pH 7.5, 50°C | Volvariella volvacea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
x * 39900, calculated from sequence | Volvariella volvacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Volvariella volvacea | Q09IZ4 | - |
- |
Volvariella volvacea V14 | Q09IZ4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Volvariella volvacea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Volvariella volvacea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl acetate + H2O | - |
Volvariella volvacea | 4-methylumbelliferone + acetate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Volvariella volvacea V14 | 4-methylumbelliferone + acetate | - |
? | |
acetylxylan + H2O | - |
Volvariella volvacea | ? | - |
? | |
acetylxylan + H2O | - |
Volvariella volvacea V14 | ? | - |
? | |
beta-D-xylose tetraacetate + H2O | - |
Volvariella volvacea | ? | - |
? | |
beta-D-xylose tetraacetate + H2O | - |
Volvariella volvacea V14 | ? | - |
? | |
additional information | alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate | Volvariella volvacea | ? | - |
? | |
additional information | alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate | Volvariella volvacea V14 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39900, calculated from sequence | Volvariella volvacea |
Synonyms | Comment | Organism |
---|---|---|
Vvaxe1 | - |
Volvariella volvacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
hydrolysis of 4-methylumbelliferyl acetate | Volvariella volvacea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 50% of maximal activity, 70°C: about 90% of maximal activity, hydrolysis of 4-methylumbelliferyl acetate | Volvariella volvacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
hydrolysis of 4-methylumbelliferyl acetate | Volvariella volvacea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | pH 7.5: about 40% of maximal activity, pH 10: about 40% of maximal activity, hydrolysis of 4-methylumbelliferyl acetate | Volvariella volvacea |