Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris, recombinant AoAXE is secreted by the aid of fused alpha-factor secretion signal peptide | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q75P26 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme appears to possess ASn-linked high-mannose oligosaccharides | Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | recombinant AoAXE is secreted by the aid of fused alpha-factor secretion signal peptide | Aspergillus oryzae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Aspergillus oryzae | 1-naphthol + acetate | - |
? | |
acetylated birchwood xylan + H2O | - |
Aspergillus oryzae | acetate + birchwood xylan | - |
? | |
alpha-naphthyl propionate + H2O | - |
Aspergillus oryzae | 1-naphthol + propionate | - |
? | |
additional information | no activity towards acyl-chain substrates containing four or more carbon atoms, methyl esters of ferulic, caffeic or sinapic acids | Aspergillus oryzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
AoAXE | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | 35°C: about 75% of maximal activity, 55°C: about 60% of maximal activity | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
room temperature, 1 h, about 50% loss of activity | Aspergillus oryzae |
6 | 7 | room temperature, 1 h, stable | Aspergillus oryzae |
8 | - |
room temperature, 1 h, about 10% loss of activity | Aspergillus oryzae |