Literature summary for 3.1.1.72 extracted from

Taylor, E.J.; Gloster, T.M.; Turkenburg, J.P.; Vincent, F.; Brzozowski, A.M.; Dupont, C.; Shareck, F.; Centeno, M.S.; Prates, J.A.; Puchart, V.; Ferreira, L.M.; Fontes, C.M.; Biely, P.; Davies, G.J.
Structure and activity of two metal ion-dependent acetylxylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases (2006), J. Biol. Chem., 281, 10968-10975.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
fragment encoding the CE4 domain in complex with Cd2+, Mg2+ or Co2+, vapour-diffusion hanging-drop method	Acetivibrio thermocellus
fragment encoding the CE4 domain, vapour-diffusion hanging-drop method	Streptomyces lividans

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Acetivibrio thermocellus
EDTA	-	Streptomyces lividans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1	-	2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside	-	Streptomyces lividans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	metal ion-dependent enzyme, Mn2+ gives 46% of the activity with Co2+	Streptomyces lividans
Cd2+	metal ion-dependent enzyme, Mn2+ gives 6% of the activity with Co2+	Acetivibrio thermocellus
Co2+	metal ion-dependent enzyme, possesses a single metal center with a chemical preference for Co2+	Streptomyces lividans
Co2+	metal ion-dependent enzyme, possesses a single metal center with a chemical preference for Co2+	Acetivibrio thermocellus
Mn2+	metal ion-dependent enzyme, Mn2+ gives 3.5% of the activity with Co2+	Acetivibrio thermocellus
Mn2+	metal ion-dependent enzyme, Mn2+ gives 40-45% of the activity with Co2+	Streptomyces lividans
Mn2+	metal ion-dependent enzyme, Mn2+ gives 40-45% of the activity with Co2+	Acetivibrio thermocellus
additional information	completely inactive with Mg2+	Streptomyces lividans
Ni2+	metal ion-dependent enzyme, Mn2+ gives 10% of the activity with Co2+	Streptomyces lividans
Ni2+	metal ion-dependent enzyme, Mn2+ gives 35% of the activity with Co2+	Acetivibrio thermocellus
Zn2+	metal ion-dependent enzyme, Mn2+ gives 33% of the activity with Co2+	Streptomyces lividans
Zn2+	metal ion-dependent enzyme, Mn2+ gives 6% of the activity with Co2+	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	-	-	-
Streptomyces lividans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant, fragment encoding the CE4 domain	Streptomyces lividans
recombinant, fragment encoding the CE4 domain	Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O	-	Streptomyces lividans	acetate + 4-nitrophenyl-beta-D-xylopyranoside	-	?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O	-	Acetivibrio thermocellus	acetate + 4-nitrophenyl-beta-D-xylopyranoside	-	?
4-nitrophenyl acetate + H2O	-	Streptomyces lividans	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	-	Acetivibrio thermocellus	4-nitrophenol + acetate	-	?

Synonyms

Synonyms	Comment	Organism
SlCE4	-	Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
118	-	2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside	-	Streptomyces lividans

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Release 2023.1 (January 2023)