Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not affected by 2-mercaptoethanol and EDTA | Termitomyces clypeatus |
General Stability | Organism |
---|---|
the enzyme aggregated with cellobiase shows higher thermal stability, temperature optimum, and resistance to chemical denaturants than the isolated purified enzyme | Termitomyces clypeatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidinium hydrochloride | 3.5-4.0 M, inactivation | Termitomyces clypeatus | |
additional information | no inhibition by xylose and acetate, enzyme is not affected by 2-mercaptoethanol and EDTA | Termitomyces clypeatus | |
SDS | 0.5% inhibit the single enzyme, but 5% do not affect the aggregated enzyme | Termitomyces clypeatus | |
Trypsin | single enzyme loses its activity completely after 30 min at 37°C, while the aggregated enzyme shows 75-80% remaining activity after 60 min incubation at 37°C | Termitomyces clypeatus | |
Urea | 7.0 M, inactivation | Termitomyces clypeatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
4-nitrophenyl acetate | pH 6.5, 50°C | Termitomyces clypeatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Termitomyces clypeatus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | about 25% activation | Termitomyces clypeatus | |
Mg2+ | about 25% activation | Termitomyces clypeatus | |
additional information | enzyme is not affected by Ca2+, Cu2+, and Zn2+ | Termitomyces clypeatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is aggregated with a cellobiase tetramer to form a 80 kDa protein complex | Termitomyces clypeatus |
30000 | - |
gel filtration | Termitomyces clypeatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Termitomyces clypeatus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Termitomyces clypeatus |
Purification (Comment) | Organism |
---|---|
purification of the enzyme as a single 30 kDa protein, 60fold, and aggregated with cellobiase to a 80 kDa protein complex | Termitomyces clypeatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. | Termitomyces clypeatus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Termitomyces clypeatus |
80 | - |
purified single enzyme | Termitomyces clypeatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Termitomyces clypeatus | 4-nitrophenol + acetate | - |
? | |
acetylated xylan + H2O | substrate from birchwood | Termitomyces clypeatus | xylan + acetate | - |
? | |
additional information | alpha-naphthyl acetate is a poor substrate | Termitomyces clypeatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Termitomyces clypeatus |
Synonyms | Comment | Organism |
---|---|---|
acetyl (xylan) esterase | - |
Termitomyces clypeatus |
Acetyl esterase | - |
Termitomyces clypeatus |
AE | - |
Termitomyces clypeatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the temperature optimum is increased by aggregation of the enzyme with cellobiase | Termitomyces clypeatus |
45 | - |
single enzyme | Termitomyces clypeatus |
55 | - |
aggregated enzyme | Termitomyces clypeatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme aggregated with cellobiase shows higher thermal stability than the isolated purified enzyme | Termitomyces clypeatus |
35 | - |
single enzyme | Termitomyces clypeatus |
50 | - |
30 min, pH 6.5, single enzyme, complete loss of activity | Termitomyces clypeatus |
70 | - |
aggregated enzyme | Termitomyces clypeatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Termitomyces clypeatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Termitomyces clypeatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Termitomyces clypeatus | chromatocfocusing | 7.4 | 7.3 |