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Literature summary for 3.1.1.72 extracted from
- Transacetylations to carbohydrates catalyzed by acetylxylan esterase in the presence of organic solvent (2003), Biochim. Biophys. Acta, 1623, 62-71.
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | enzyme has a half-life of 1 h in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water | Schizophyllum commune |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizophyllum commune | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. | Schizophyllum commune |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 63 | - |
purified enzyme | Schizophyllum commune |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0.1 M sodium phosphate, NaN3, several years | Schizophyllum commune |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (glucose)4 + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? |  |
| (mannose)2 + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| (mannose)5 + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| (mannose)6 + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| (xylose)2 + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| (xylose)5 + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Schizophyllum commune | 4-nitrophenol + acetate | - |
? | |
| methyl beta-cellobiose + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| methyl beta-cellobiose + vinyl acetate | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| methyl beta-xylopyranoside + triacetin | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| methyl beta-xylopyranoside + vinyl acetate | transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity | Schizophyllum commune | ? | - |
? | |
| additional information | formation of monoacetates 4-, 3-, and 2-O-acetyl-methyl-beta-xylosepyranoside in a ratio 1:0.9:0.6, also of diacetates 2,3-, 3,4-, and 2,4-di-O-acetyl-methyl-beta-xylosepyranoside in a ratio 1:0.47:0.28 | Schizophyllum commune | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcXE | - |
Schizophyllum commune |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at, room temperature | Schizophyllum commune |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Schizophyllum commune |
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Release 2023.1 (January 2023)
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