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Literature summary for 3.1.1.72 extracted from

  • Benini, S.; Degrassi, G.; Krastanova, I.; Lamba, D.; Venturi, V.
    Purification, crystallization and preliminary X-ray analysis of an acetylxylan esterase from Bacillus pumilus (2001), Acta Crystallogr. Sect. D, 57, 1906-1907.
    View publication on PubMed
Search for more literature for 3.1.1.72

Activating Compound

Activating Compound Comment Organism Structure
corncob in the medium, induces enzyme production Bacillus pumilus
xylan induction of enzyme production Bacillus pumilus

Application

Application Comment Organism
medicine possible application of the enzyme in antibiotic production, e.g. in the deacetylation of cephalosporin C Bacillus pumilus

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha Bacillus pumilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36012
-
 6 * 36012, amino acid calculation and crystal structure Bacillus pumilus
190000
-
 about, gel filtration Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli DH5alpha, preparation still contains traces of Escherichia coli inorganic pyrophosphatase Bacillus pumilus

Reaction

Reaction Comment Organism Reaction ID
acetylxylan + 3 H2O = 3 acetic acid + xylan catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin, enzyme possesses a catalytic triad of Ser-His-Asp, catalytic mechanism Bacillus pumilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Bacillus pumilus 4-nitrophenol + acetate
-
 ?
acetylated xylan + H2O
-
 Bacillus pumilus xylan + acetate
-
 ?
cephalosporin C + H2O
-
 Bacillus pumilus deacetylcephalosporin C + acetate
-
 ?
D-glucose pentaacetate + H2O
-
 Bacillus pumilus D-glucose + acetate
-
 ?
xylose tetraacetate + H2O
-
 Bacillus pumilus xylose + acetate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 36012, amino acid calculation and crystal structure Bacillus pumilus

Synonyms

Synonyms Comment Organism
AXE
-
 Bacillus pumilus