Cloned (Comment) | Organism |
---|---|
gene ache, recombinant expression of SUMO-His6-tagged human brain acetylcholinesterase in Escherichia coli strain BL21(DE3), method evaluation, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
acetylthiocholine | pH 8.0, 22°C, recombinant SUMO-His6-tagged enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Homo sapiens | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P22303 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | glycosylation is required for membrane trafficking. When considered in this regard, the use of Escherichia coli seems to be compatible for recombinant hAChE production | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant SUMO-His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? | |
acetylthiocholine + H2O | activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate | Homo sapiens | thiocholine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, about, recombinant SUMO-His6-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Homo sapiens |
hAChE | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, recombinant SUMO-His6-tagged enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant SUMO-His6-tagged enzyme | Homo sapiens |