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Literature summary for 3.1.1.7 extracted from

  • Mohamed, M.A.; M E Mahdy, E.S.; Ghazy, A.M.; Ibrahim, N.M.; El-Mezayen, H.A.; Ghanem, M.M.
    Acetylcholinesterases from entomopathogenic nematode Heterorhabditid bacteriophora susceptibility to insecticides and immunological characteristics (2017), Pestic. Biochem. Physiol., 135, 27-34 .
    View publication on PubMed
Search for more literature for 3.1.1.7

Inhibitors

Inhibitors Comment Organism Structure
acetamiprid complete inhibition Heterorhabditis bacteriophora
carbofuran complete inhibition Heterorhabditis bacteriophora
malathion non-competitive inhibition Heterorhabditis bacteriophora
methomyl complete inhibition Heterorhabditis bacteriophora
additional information AChE isoenzymes, AChEAII and AChEBI isolated from Heterorhabditid bacteriophora EM2 strain, show insensitivity to inhibition by insecticides in a descending order: methomyl, carbofuran, acetamiprid, oxamyl, malathion. The two AChE isoforms are several folds less sensitive to inhibition by methomyl and carbofuran compared to those previously reported for other insect species. Except for malathion, all the insecticides competitively inhibit Heterorhabditid bacteriophora AChEs with Ki values ranging from 0.10 to 15 mM Heterorhabditis bacteriophora
oxamyl complete inhibition Heterorhabditis bacteriophora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Heterorhabditis bacteriophora
-
 choline + acetate
-
 ?
acetylcholine + H2O Heterorhabditis bacteriophora EM2
-
 choline + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Heterorhabditis bacteriophora
-
-
-
Heterorhabditis bacteriophora EM2
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
 total activity of AChE in nematodes of strain EM2, pH 8.5, 37°C Heterorhabditis bacteriophora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
 Heterorhabditis bacteriophora choline + acetate
-
 ?
acetylcholine + H2O
-
 Heterorhabditis bacteriophora EM2 choline + acetate
-
 ?
acetylthiocholine + H2O
-
 Heterorhabditis bacteriophora thiocholine + acetate
-
 ?
acetylthiocholine + H2O
-
 Heterorhabditis bacteriophora EM2 thiocholine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
AChE
-
 Heterorhabditis bacteriophora
AChEAII
-
 Heterorhabditis bacteriophora
AChEBI
-
 Heterorhabditis bacteriophora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 isozymes AChEAII and AChEBI Heterorhabditis bacteriophora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 isozymes AChEAII and AChEBI Heterorhabditis bacteriophora

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics analysis Heterorhabditis bacteriophora
0.063
-
 malathion pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora
0.075
-
 malathion pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora
0.1
-
 oxamyl pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora
0.12
-
 oxamyl pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora
0.75
-
 acetamiprid pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora
0.85
-
 acetamiprid pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora
1.25
-
 carbofuran pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora
2.5
-
 carbofuran pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora
12.5
-
 methomyl pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora
15
-
 methomyl pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.087
-
 pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora malathion
0.11
-
 pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora malathion
0.16
-
 pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora oxamyl
0.21
-
 pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora oxamyl
1.23
-
 pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora acetamiprid
1.4
-
 pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora carbofuran
1.64
-
 pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora acetamiprid
2.57
-
 pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora carbofuran
18
-
 pH 8.5, 37°C, isozyme AChEBI Heterorhabditis bacteriophora methomyl
23
-
 pH 8.5, 37°C, isozyme AChEAII Heterorhabditis bacteriophora methomyl