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Literature summary for 3.1.1.7 extracted from
- Characterization of butyrylcholinesterase from porcine milk (2018), Arch. Biochem. Biophys., 652, 38-49 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of HuAChE in CHO cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (-)-huperzine A | - |
Homo sapiens |  |
| 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | i.e. BW284c51 | Homo sapiens | |
| acetylthiocholine | - |
Homo sapiens | |
| butyrylthiocholine | - |
Homo sapiens | |
| decamethonium | - |
Homo sapiens | |
| diisopropyl fluorophosphate | inhibition, aging, and reactivation kinetics | Homo sapiens | |
| edrophonium | - |
Homo sapiens | |
| ethopropazine | - |
Homo sapiens | |
| additional information | recombinant HuAChE shows substrate inhibition | Homo sapiens | |
| phenothiazine | - |
Homo sapiens | |
| propidium | - |
Homo sapiens | |
| propionylthiocholine | - |
Homo sapiens | |
| tacrine | - |
Homo sapiens | |
| tetra(monoisopropyl)diphosphortetramide | irreversible | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 0.15 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | Homo sapiens | - |
choline + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P22303 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme HuAChE from CHO cells by procainamide affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? | |
| acetylthiocholine + H2O | - |
Homo sapiens | thiocholine + acetate | - |
? | |
| additional information | no activity with butyrylcholine | Homo sapiens | ? | - |
? | |
| propionylthiocholine + H2O | - |
Homo sapiens | thiocholine + propionate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AChE | - |
Homo sapiens |
| HuAChE | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.72 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 7.18 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000032 | - |
(-)-huperzine A | pH 8.0, 22°C | Homo sapiens | |
| 0.000003 | - |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | pH 8.0, 22°C | Homo sapiens | |
| 0.000037 | - |
tacrine | pH 8.0, 22°C | Homo sapiens | |
| 0.00036 | - |
edrophonium | pH 8.0, 22°C | Homo sapiens | |
| 0.00049 | - |
propidium | pH 8.0, 22°C | Homo sapiens | |
| 0.0022 | - |
decamethonium | pH 8.0, 22°C | Homo sapiens | |
| 0.0046 | - |
tetra(monoisopropyl)diphosphortetramide | pH 8.0, 22°C | Homo sapiens | |
| 0.0653 | - |
ethopropazine | pH 8.0, 22°C | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the concentration of active sites in rHuAChE is determined by titration with 7-(O,O-diethyl-phosphinyloxy)-1-methylquinolinium methylsulfate (DEPQ) | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24.8 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 65.3 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
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