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Literature summary for 3.1.1.7 extracted from

  • Lu, Y.H.; He, Y.P.; Gao, X.W.
    Comparative studies on acetylcholinesterase characteristics between the aphids, Sitobion avenae and Rhopalosiphum padi (2013), J. Insect Sci., 13, 9-9.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.7

Inhibitors

Inhibitors Comment Organism Structure
BW284c51 highly sensitive to inhibition by BW284C51 Rhopalosiphum padi
BW284c51 highly sensitive to inhibition by BW284C51 Sitobion avenae
eserine highly sensitive to inhibition by eserine (20% residual activity at 0.01 mM) Rhopalosiphum padi
eserine highly sensitive to inhibition by eserine (10% residual activity at 0.01 mM) Sitobion avenae
methomyl
-
 Rhopalosiphum padi
methomyl
-
 Sitobion avenae
pirimicarb
-
 Rhopalosiphum padi
pirimicarb
-
 Sitobion avenae
thiodicarb
-
 Rhopalosiphum padi
thiodicarb
-
 Sitobion avenae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 butylthiocholine purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi
0.85
-
 propylthiocholine purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi
0.93
-
 propylthiocholine purified enzyme, at pH 7.5 and 30°C Sitobion avenae
1.69
-
 butylthiocholine purified enzyme, at pH 7.5 and 30°C Sitobion avenae
2.18
-
 acetylthiocholine purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi
2.23
-
 methylthiocholine purified enzyme, at pH 7.5 and 30°C Sitobion avenae
2.28
-
 acetylthiocholine purified enzyme, at pH 7.5 and 30°C Sitobion avenae
2.45
-
 methylthiocholine purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Rhopalosiphum padi
-
 choline + acetate
-
 ?
acetylcholine + H2O Sitobion avenae
-
 choline + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhopalosiphum padi
-
-
-
Sitobion avenae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
procainamide-based Sepharose 4B column chromatography and Sephadex G-25 gel filtration Rhopalosiphum padi
procainamide-based Sepharose 4B column chromatography and Sephadex G-25 gel filtration Sitobion avenae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0177
-
 with methylthiocholine as substrate, purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi
0.0203
-
 with methylthiocholine as substrate, purified enzyme, at pH 7.5 and 30°C Sitobion avenae
0.0704
-
 with acetylthiocholine as substrate, purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi
0.0986
-
 with acetylthiocholine as substrate, purified enzyme, at pH 7.5 and 30°C Sitobion avenae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
 Rhopalosiphum padi choline + acetate
-
 ?
acetylcholine + H2O
-
 Sitobion avenae choline + acetate
-
 ?
acetylthiocholine + H2O
-
 Rhopalosiphum padi thiocholine + acetate
-
 ?
acetylthiocholine + H2O
-
 Sitobion avenae thiocholine + acetate
-
 ?
butylthiocholine + H2O
-
 Rhopalosiphum padi thiocholine + butanol
-
 ?
butylthiocholine + H2O
-
 Sitobion avenae thiocholine + butanol
-
 ?
methylthiocholine + H2O
-
 Rhopalosiphum padi thiocholine + methanol
-
 ?
methylthiocholine + H2O
-
 Sitobion avenae thiocholine + methanol
-
 ?
propylthiocholine + H2O
-
 Rhopalosiphum padi thiocholine + propanol
-
 ?
propylthiocholine + H2O
-
 Sitobion avenae thiocholine + propanol
-
 ?

Synonyms

Synonyms Comment Organism
AChE
-
 Rhopalosiphum padi
AChE
-
 Sitobion avenae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00093
-
 purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi thiodicarb
0.001
-
 purified enzyme, at pH 7.5 and 30°C Sitobion avenae BW284c51
0.00159
-
 purified enzyme, at pH 7.5 and 30°C Sitobion avenae thiodicarb
0.00164
-
 purified enzyme, at pH 7.5 and 30°C Sitobion avenae eserine
0.00189
-
 purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi eserine
0.00226
-
 purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi pirimicarb
0.00257
-
 purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi BW284c51
0.00351
-
 purified enzyme, at pH 7.5 and 30°C Sitobion avenae pirimicarb
0.00556
-
 purified enzyme, at pH 7.5 and 30°C Rhopalosiphum padi methomyl
0.00723
-
 purified enzyme, at pH 7.5 and 30°C Sitobion avenae methomyl