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Literature summary for 3.1.1.7 extracted from

  • Kim, Y.H.; Choi, J.Y.; Je, Y.H.; Koh, Y.H.; Lee, S.H.
    Functional analysis and molecular characterization of two acetylcholinesterases from the German cockroach, Blattella germanica (2010), Insect Mol. Biol., 19, 765-776.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a recombinant baculovirus expression system Blattella germanica

Inhibitors

Inhibitors Comment Organism Structure
2,2-dichlorovinyl dimethyl phosphate
-
Blattella germanica
BW284c51
-
Blattella germanica
Carbaryl
-
Blattella germanica
eserine
-
Blattella germanica
additional information not inhibited by iso-OMPA Blattella germanica
paraoxon
-
Blattella germanica
propoxur
-
Blattella germanica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
butyrylthiocholine iodide recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica
0.067
-
acetylthiocholine iodide recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica
0.111
-
acetylthiocholine iodide recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica
0.182
-
butyrylthiocholine iodide recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
2 * 73000, isoform AChE2, SDS-PAGE Blattella germanica
80000
-
2 * 80000, isoform AChE1, SDS-PAGE Blattella germanica
146000
-
isoform AChE2, nonreducing PAGE Blattella germanica
160000
-
isoform AChE1, nonreducing PAGE Blattella germanica

Organism

Organism UniProt Comment Textmining
Blattella germanica
-
German cockroach
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein most fractions of isoform AChE1 have a glycophosphatidylinositol anchor, but a small fraction comprises a collagen-like tail. Isoform AChE2 has a collagen-glycophosphatidylinositoI-fused tail Blattella germanica

Source Tissue

Source Tissue Comment Organism Textmining
abdomen
-
Blattella germanica
-
ganglion highest AChE1 activity Blattella germanica
-
gut
-
Blattella germanica
-
head
-
Blattella germanica
-
leg
-
Blattella germanica
-
thorax
-
Blattella germanica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine iodide + H2O
-
Blattella germanica thiocholine iodide + acetate
-
?
butyrylthiocholine iodide + H2O
-
Blattella germanica thiocholine iodide + butyrate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 73000, isoform AChE2, SDS-PAGE Blattella germanica
homodimer 2 * 80000, isoform AChE1, SDS-PAGE Blattella germanica

Synonyms

Synonyms Comment Organism
acetylcholinesterase 1
-
Blattella germanica
acetylcholinesterase 2
-
Blattella germanica
AChE
-
Blattella germanica
AChE1 isoform Blattella germanica
AChE2 isoform Blattella germanica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000298
-
recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica eserine
0.000368
-
recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica BW284c51
0.000441
-
recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica BW284c51
0.00133
-
recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica paraoxon
0.00201
-
recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica eserine
0.00335
-
recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica 2,2-dichlorovinyl dimethyl phosphate
0.00447
-
recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica paraoxon
0.00755
-
recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica propoxur
0.00799
-
recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica propoxur
0.0135
-
recombinant enzyme AChE2, pH not specified in the publication, 30°C Blattella germanica Carbaryl
0.0861
-
recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica 2,2-dichlorovinyl dimethyl phosphate
0.36
-
recombinant enzyme AChE1, pH not specified in the publication, 30°C Blattella germanica Carbaryl

General Information

General Information Comment Organism
physiological function isoform AChE1 plays a major role in postsynaptic transmission Blattella germanica