Cloned (Comment) | Organism |
---|---|
expressed in a recombinant baculovirus expression system | Blattella germanica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2-dichlorovinyl dimethyl phosphate | - |
Blattella germanica | |
BW284c51 | - |
Blattella germanica | |
Carbaryl | - |
Blattella germanica | |
eserine | - |
Blattella germanica | |
additional information | not inhibited by iso-OMPA | Blattella germanica | |
paraoxon | - |
Blattella germanica | |
propoxur | - |
Blattella germanica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
butyrylthiocholine iodide | recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | |
0.067 | - |
acetylthiocholine iodide | recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | |
0.111 | - |
acetylthiocholine iodide | recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica | |
0.182 | - |
butyrylthiocholine iodide | recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
2 * 73000, isoform AChE2, SDS-PAGE | Blattella germanica |
80000 | - |
2 * 80000, isoform AChE1, SDS-PAGE | Blattella germanica |
146000 | - |
isoform AChE2, nonreducing PAGE | Blattella germanica |
160000 | - |
isoform AChE1, nonreducing PAGE | Blattella germanica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blattella germanica | - |
German cockroach | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | most fractions of isoform AChE1 have a glycophosphatidylinositol anchor, but a small fraction comprises a collagen-like tail. Isoform AChE2 has a collagen-glycophosphatidylinositoI-fused tail | Blattella germanica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
abdomen | - |
Blattella germanica | - |
ganglion | highest AChE1 activity | Blattella germanica | - |
gut | - |
Blattella germanica | - |
head | - |
Blattella germanica | - |
leg | - |
Blattella germanica | - |
thorax | - |
Blattella germanica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine iodide + H2O | - |
Blattella germanica | thiocholine iodide + acetate | - |
? | |
butyrylthiocholine iodide + H2O | - |
Blattella germanica | thiocholine iodide + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 73000, isoform AChE2, SDS-PAGE | Blattella germanica |
homodimer | 2 * 80000, isoform AChE1, SDS-PAGE | Blattella germanica |
Synonyms | Comment | Organism |
---|---|---|
acetylcholinesterase 1 | - |
Blattella germanica |
acetylcholinesterase 2 | - |
Blattella germanica |
AChE | - |
Blattella germanica |
AChE1 | isoform | Blattella germanica |
AChE2 | isoform | Blattella germanica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000298 | - |
recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | eserine | |
0.000368 | - |
recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | BW284c51 | |
0.000441 | - |
recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica | BW284c51 | |
0.00133 | - |
recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica | paraoxon | |
0.00201 | - |
recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica | eserine | |
0.00335 | - |
recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | 2,2-dichlorovinyl dimethyl phosphate | |
0.00447 | - |
recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | paraoxon | |
0.00755 | - |
recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica | propoxur | |
0.00799 | - |
recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | propoxur | |
0.0135 | - |
recombinant enzyme AChE2, pH not specified in the publication, 30°C | Blattella germanica | Carbaryl | |
0.0861 | - |
recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica | 2,2-dichlorovinyl dimethyl phosphate | |
0.36 | - |
recombinant enzyme AChE1, pH not specified in the publication, 30°C | Blattella germanica | Carbaryl |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform AChE1 plays a major role in postsynaptic transmission | Blattella germanica |