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Literature summary for 3.1.1.7 extracted from
- Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme (2006), Invert. Neurosci., 6, 47-55.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in COS-7 cells | Tetronarce californica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C310A | site-directed mutagenesis, the mutant shows altered sulfhydryl reagents binding and inactivation, overview | Tetronarce californica |
| C310A/C466A | site-directed mutagenesis, the mutant shows altered sulfhydryl reagents binding and inactivation, overview | Tetronarce californica |
| C310A/F312I | site-directed mutagenesis, the mutant shows altered sulfhydryl reagents binding and inactivation, overview | Tetronarce californica |
| C466A | site-directed mutagenesis, the mutant shows altered sulfhydryl reagents binding and inactivation, overview | Tetronarce californica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | inactivation, the peripheral site ligand propidium accelerates inactivation in the wild type ChE2, but retards inactivation in the F312I mutant | Tetronarce californica |  |
| additional information | binding structure of sulfhydryl reagents to AChE, overview | Tetronarce californica | |
| N-ethylmaleimide | inactivation | Tetronarce californica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetronarce californica | P04058 | isozyme ChE2 | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AChE | - |
Tetronarce californica |
| ChE2 | - |
Tetronarce californica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Tetronarce californica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Tetronarce californica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inactivation by DTNB and NEM follows first-order kinetics | Tetronarce californica |
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Release 2023.1 (January 2023)
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