Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.7 extracted from

  • Colletier, J.P.; Fournier, D.; Greenblatt, H.M.; Stojan, J.; Sussman, J.L.; Zaccai, G.; Silman, I.; Weik, M.
    Structural insights into substrate traffic and inhibition in acetylcholinesterase (2006), EMBO J., 25, 2746-2756.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme by hanging-drop vapour diffusion method, 4°C, 11.5 mg/ml protein, mother liquor is 32% PEG 200 in 0.15 M MES, pH 5.8-6.2, soaking of crystals at 4°C in mother liquor at pH 6.0, containing either 0.5 mM OTMA for 24 h in 5 ml soaking solution, 20 mM TCh for 12 h in 5 ml soaking solution, 500 mM ATCh for 40 min in 1.5 ml soaking solution, or 20 mM ATCh for 12 h in 200 ml soaking solution, X-ray diffraction structure determination and anaylsis at 2.0-2.7 A resolution Tetronarce californica

Protein Variants

Protein Variants Comment Organism
E199D site-directed mutagenesis, the substrate inhibition is abolished in the mutant enzyme Tetronarce californica

Inhibitors

Inhibitors Comment Organism Structure
4-oxo-N,N,N-trimethylpentanaminium iodide i.e. OTMA, a hydrolysable substrate analogue, binding structure analysis Tetronarce californica
Acetylcholine substrate inhibition due to choline exit being hindered by a substrate molecule bound at the peripheral site Tetronarce californica
acetylthiocholine interaction with Glu199 of ATCh, substrate inhibition due to choline exit being hindered by a substrate molecule bound at the peripheral site Tetronarce californica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Tetronarce californica
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Tetronarce californica P04058
-
-

Reaction

Reaction Comment Organism Reaction ID
acetylcholine + H2O = choline + acetate reaction mechanism, structure-function relationship analysis, binding structure and mechanism of substrate and product at both the peripheral and active sites, overview Tetronarce californica

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Tetronarce californica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Tetronarce californica choline + acetate
-
?
acetylcholine + H2O substrate traffic in AChE involves at least two binding sites, the catalytic and peripheral anionic sites, which are allosterically related and involved in substrate inhibition, structure-function relationship analysis, overview Tetronarce californica choline + acetate
-
?
acetylthiocholine iodide + H2O
-
Tetronarce californica thiocholine iodide + acetate
-
?

Subunits

Subunits Comment Organism
More modelling of molecular surfaces of the active-site gorge in the native enzyme, overview Tetronarce californica

Synonyms

Synonyms Comment Organism
AChE
-
Tetronarce californica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Tetronarce californica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Tetronarce californica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics, data are fitted by multiple nonlinear regressions using a model that assumes two substrate-inhibition mechanisms, overview Tetronarce californica