Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BW284c51 | isozyme AChE1A, complete competitive inhibition at 0.016 mM | Heterorhabditis bacteriophora | |
eserine | isozyme AChE1A, complete competitive inhibition at 0.016 mM | Heterorhabditis bacteriophora | |
additional information | no inhibition by iso-OMPA | Heterorhabditis bacteriophora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | MIchaelis-Menten kinetics, activation energy of 9 kcal/mol | Heterorhabditis bacteriophora | |
0.27 | - |
acetylthiocholine iodide | pH 7.5, 37°C, isozyme AChE1A | Heterorhabditis bacteriophora | |
0.42 | - |
propionylthiocholine iodide | pH 7.5, 37°C, isozyme AChE1A | Heterorhabditis bacteriophora | |
0.59 | - |
butyrylthiocholine iodide | pH 7.5, 37°C, isozyme AChE1A | Heterorhabditis bacteriophora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Heterorhabditis bacteriophora | - |
eight different Egyptian strains EA2, EB6, EB7, EG7, EG8, EG9, EK1 and EK2, different isozymes, i.e. AChE1A, isozyme pattern | - |
Purification (Comment) | Organism |
---|---|
isozyme AChE1A from strain EG9, 17.5fold from infective juveniles, by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography to homogeneity | Heterorhabditis bacteriophora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
juvenile | infective | Heterorhabditis bacteriophora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
strain EG7 | Heterorhabditis bacteriophora |
44.2 | - |
strain EB7 | Heterorhabditis bacteriophora |
59.1 | - |
strain EA2 | Heterorhabditis bacteriophora |
59.9 | - |
strain EK2 and EK1 | Heterorhabditis bacteriophora |
60.1 | - |
strain EG8 | Heterorhabditis bacteriophora |
71.5 | - |
strain EB6 | Heterorhabditis bacteriophora |
82.6 | - |
strain EG9 | Heterorhabditis bacteriophora |
1378 | - |
purified isozyme AChE1A from strain EG9 | Heterorhabditis bacteriophora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine iodide + H2O | preferred substrate | Heterorhabditis bacteriophora | thiocholine iodide + acetate | - |
? | |
benzoylthiocholine iodide + H2O | 22.2% activity compared to acetylthiocholine | Heterorhabditis bacteriophora | thiocholine iodide + benzoate | - |
? | |
butyrylthiocholine iodide + H2O | 41.7% activity compared to acetylthiocholine | Heterorhabditis bacteriophora | thiocholine iodide + butyrate | - |
? | |
propionylthiocholine iodide + H2O | 74.6% activity compared to acetylthiocholine | Heterorhabditis bacteriophora | thiocholine iodide + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Heterorhabditis bacteriophora |
AChE1A | - |
Heterorhabditis bacteriophora |
ChE1A | - |
Heterorhabditis bacteriophora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Heterorhabditis bacteriophora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified isozyme AChE1A, irreversible inactivation above | Heterorhabditis bacteriophora |
60 | - |
30 min, purified isozyme AChE1A, loss of 46% activity | Heterorhabditis bacteriophora |
70 | - |
30 min, purified isozyme AChE1A, loss of 63% activity | Heterorhabditis bacteriophora |
80 | - |
30 min, purified isozyme AChE1A, loss of 82% activity | Heterorhabditis bacteriophora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Heterorhabditis bacteriophora |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
BW284c51 | pH 7.5, 37°C, isozyme AChE1A | Heterorhabditis bacteriophora | |
0.005 | - |
eserine | pH 7.5, 37°C, isozyme AChE1A | Heterorhabditis bacteriophora |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.003 | - |
pH 7.5, 37°C, isozyme AChE1A | Heterorhabditis bacteriophora | BW284c51 | |
0.0035 | - |
pH 7.5, 37°C, isozyme AChE1A | Heterorhabditis bacteriophora | eserine |