Literature summary for 3.1.1.7 extracted from

Colletier, J.P.; Royant, A.; Specht, A.; Sanson, B.; Nachon, F.; Masson, P.; Zaccai, G.; Sussman, J.L.; Goeldner, M.; Silman, I.; Bourgeois, D.; Weik, M.
Use of a caged analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography (2007), Acta Crystallogr. Sect. D, 63, 1115-1128.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hangingdrop vapour-diffusion method, 4°C, 11.5 mg/ml protein, the mother liquor contains 32% PEG 200, 0.15 M MES, pH 5.8-6.2, soaking of the crystals in the dark for 12 h at 4°C in mother liquor containing 5 mM [1-(2-nitrophenyl)-2,2,2-trifluoroethyl]-arsenocholine iodide, pH 6.0, X-ray diffraction structure determination and anaylsis at 2.4 A resolution	Tetronarce californica

Inhibitors

Inhibitors	Comment	Organism	Structure
[1-(2-nitrophenyl)-2,2,2-trifluoroethyl]-arsenocholine iodide	caged compound, binding structure and mechanism with AChE, binding within the active-site gorge, overview	Tetronarce californica

Organism

Organism	UniProt	Comment	Textmining
Tetronarce californica	P04058	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetylthiocholine + H2O	catalytic residues are Ser200 and Trp84	Tetronarce californica	thiocholine + acetate	-	?

Subunits

Subunits	Comment	Organism
More	two domains on either side of the active-site gorge, residues 4-305 and residues 306-535, the catalytic Ser200 and Trp84 are shown at the bottom of the gorge and the peripheral-site Trp279 at its rim, three-dimensional structure, overview	Tetronarce californica

Synonyms

Synonyms	Comment	Organism
AChE	-	Tetronarce californica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Tetronarce californica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Tetronarce californica

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.027	-	[1-(2-nitrophenyl)-2,2,2-trifluoroethyl]-arsenocholine iodide	pH 7.0, 20°C	Tetronarce californica

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Release 2023.1 (January 2023)