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Literature summary for 3.1.1.7 extracted from

  • Sagane, Y.; Nakagawa, T.; Yamamoto, K.; Michikawa, S.; Oguri, S.; Momonoki, Y.S.
    Molecular characterization of maize acetylcholinesterase: a novel enzyme family in the plant kingdom (2005), Plant Physiol., 138, 1359-1371.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Zea mays

Inhibitors

Inhibitors Comment Organism Structure
neostigmine bromide competitive Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
propionylthiocholine pH 7.0, 30°C Zea mays
4.7
-
acetylthiocholine pH 7.0, 30°C Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide Zea mays

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82.5
-
pH 7.0, 30°C Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine + H2O
-
Zea mays acetate + thiocholine
-
?
additional information no substrate: S-butyrylthiocholine Zea mays ?
-
?
propionylthiocholine + H2O preferred over acetylthiocholine Zea mays propanoate + thiocholine
-
?

Subunits

Subunits Comment Organism
? enzyme exists as a mixture of 88 kDa dimers consisting of disulfide-linked 42- to 44-kDa polypeptides and a 42- to 44-kDa monomer, SDS-PAGE Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0031
-
neostigmine bromide pH 7.0, 30°C, substrate acetylthiocholine Zea mays
0.0047
-
neostigmine bromide pH 7.0, 30°C, substrate propionylthiocholine Zea mays