Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
low molecular weight minor protein fraction in liver microsomes with FAEES activity, gel filtration | Rattus norvegicus |
180000 | - |
high molecular weight major protein fraction in liver microsomes with FAEES activity, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
5 distinct 60 kDa low molecular weight proteins, 10.2-32.5fold | Rattus norvegicus |
from liver microsomes, 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0111 | - |
60 kDa low molecular weight protein from liver microsomes with pI 5.3 | Rattus norvegicus |
0.0115 | - |
60 kDa low molecular weight protein from liver microsomes with pI 6.5 | Rattus norvegicus |
0.0125 | - |
60 kDa low molecular weight protein from liver microsomes with pI 5.0 | Rattus norvegicus |
0.0133 | - |
60 kDa low molecular weight protein from liver microsomes with pI 5.6 | Rattus norvegicus |
0.035 | - |
60 kDa low molecular weight protein from liver microsomes with pI 5.8 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fatty acid + ethanol | - |
Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
additional information | structural and functional relationship to hepatic microsomal carboxylesterases, low molecular weight FAEE synthase activity most probably is expressed by various isozymes of hepatic microsomal carboxylesterases, which are also involved in the biotransformation of xenobiotic alcohols and amines, the LMW protein fraction also exhibits fatty acid 2-chloroethyl ester synthase and p-nitrophenyl acetate-hydrolyzing activity | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, low molecular weight minor protein fraction, SDS-PAGE | Rattus norvegicus |
trimer | 3 * 60000, high molecular weight major protein fraction, SDS-PAGE | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing | - |
5 |
Rattus norvegicus | one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing | - |
5.3 |
Rattus norvegicus | one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing | 5.9 | 5.6 |
Rattus norvegicus | one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing | - |
5.8 |
Rattus norvegicus | one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing | - |
6.5 |