BRENDA - Enzyme Database show
show all sequences of 3.1.1.67

Purification and characterization of rat hepatic microsomal low molecular weight fatty acid ethyl ester synthase and its relationship to carboxylesterases

Kaphalia, B.S.; Ansari, G.A.S.; J. Biochem. Mol. Toxicol. 15, 165-171 (2001)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity
Rattus norvegicus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
low molecular weight minor protein fraction in liver microsomes with FAEES activity, gel filtration
Rattus norvegicus
180000
-
high molecular weight major protein fraction in liver microsomes with FAEES activity, gel filtration
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
5 distinct 60 kDa low molecular weight proteins, 10.2-32.5fold; from liver microsomes, 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0111
-
60 kDa low molecular weight protein from liver microsomes with pI 5.3
Rattus norvegicus
0.0115
-
60 kDa low molecular weight protein from liver microsomes with pI 6.5
Rattus norvegicus
0.0125
-
60 kDa low molecular weight protein from liver microsomes with pI 5.0
Rattus norvegicus
0.0133
-
60 kDa low molecular weight protein from liver microsomes with pI 5.6
Rattus norvegicus
0.035
-
60 kDa low molecular weight protein from liver microsomes with pI 5.8
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fatty acid + ethanol
-
646074
Rattus norvegicus
fatty acyl ethyl ester + H2O
-
646074
Rattus norvegicus
?
additional information
structural and functional relationship to hepatic microsomal carboxylesterases, low molecular weight FAEE synthase activity most probably is expressed by various isozymes of hepatic microsomal carboxylesterases, which are also involved in the biotransformation of xenobiotic alcohols and amines, the LMW protein fraction also exhibits fatty acid 2-chloroethyl ester synthase and p-nitrophenyl acetate-hydrolyzing activity
646074
Rattus norvegicus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 60000, low molecular weight minor protein fraction, SDS-PAGE
Rattus norvegicus
trimer
3 * 60000, high molecular weight major protein fraction, SDS-PAGE
Rattus norvegicus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
5
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
5.3
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
5.9
5.6
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
5.8
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
6.5
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
low molecular weight minor protein fraction in liver microsomes with FAEES activity, gel filtration
Rattus norvegicus
180000
-
high molecular weight major protein fraction in liver microsomes with FAEES activity, gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
5 distinct 60 kDa low molecular weight proteins, 10.2-32.5fold; from liver microsomes, 180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
180 kDa high molecular weight major protein fraction and 60 kDa low molecular weight minor protein fraction in liver microsomes with FAEES activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0111
-
60 kDa low molecular weight protein from liver microsomes with pI 5.3
Rattus norvegicus
0.0115
-
60 kDa low molecular weight protein from liver microsomes with pI 6.5
Rattus norvegicus
0.0125
-
60 kDa low molecular weight protein from liver microsomes with pI 5.0
Rattus norvegicus
0.0133
-
60 kDa low molecular weight protein from liver microsomes with pI 5.6
Rattus norvegicus
0.035
-
60 kDa low molecular weight protein from liver microsomes with pI 5.8
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fatty acid + ethanol
-
646074
Rattus norvegicus
fatty acyl ethyl ester + H2O
-
646074
Rattus norvegicus
?
additional information
structural and functional relationship to hepatic microsomal carboxylesterases, low molecular weight FAEE synthase activity most probably is expressed by various isozymes of hepatic microsomal carboxylesterases, which are also involved in the biotransformation of xenobiotic alcohols and amines, the LMW protein fraction also exhibits fatty acid 2-chloroethyl ester synthase and p-nitrophenyl acetate-hydrolyzing activity
646074
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 60000, low molecular weight minor protein fraction, SDS-PAGE
Rattus norvegicus
trimer
3 * 60000, high molecular weight major protein fraction, SDS-PAGE
Rattus norvegicus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
5
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
5.3
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
5.9
5.6
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
5.8
Rattus norvegicus
one of five 60 kDa low molecular weight proteins in liver microsomes with FAEES activity, isoelectric focusing
-
6.5
Other publictions for EC 3.1.1.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750329
Tseng
-
Carboxylesterase 2 is a fatty ...
Mus musculus
ChemistrySelect
2
1516-1520
2017
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750562
De Jong
Physiological and transcripti ...
Saccharomyces cerevisiae
FEMS Yeast Res.
16
fov105
2015
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1
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679736
Bora
Alcohol linked to enhanced ang ...
Rattus norvegicus
FEBS J.
273
1403-1414
2006
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2
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1
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3
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1
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1
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1
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1
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666181
Mericle
Modulation of fatty acid methy ...
Rattus norvegicus
J. Toxicol. Environ. Health
67
583-593
2004
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-
-
-
-
-
1
-
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-
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4
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4
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1
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1
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4
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1
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666967
Kaphalia
Mechanism of differential inhi ...
Homo sapiens, Rattus norvegicus
Toxicol. Appl. Pharmacol.
200
7-15
2004
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-
-
-
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3
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5
-
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2
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2
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2
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646070
Mericle
Differential inhibition of hep ...
Rattus norvegicus
Toxicol. Appl. Pharmacol.
179
119-125
2002
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1
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2
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5
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6
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3
1
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1
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2
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6
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3
1
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646074
Kaphalia
Purification and characterizat ...
Rattus norvegicus
J. Biochem. Mol. Toxicol.
15
165-171
2001
-
-
-
-
-
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1
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2
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2
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1
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2
5
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2
2
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5
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1
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1
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2
5
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2
2
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5
-
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-
-
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646076
Diczfalusy
Characterization of enzymes in ...
Homo sapiens
J. Lipid Res.
42
1025-1032
2001
-
-
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1
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1
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2
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9
1
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1
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9
1
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1
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646075
Kaphalia
Fatty acid ethyl and methyl es ...
Homo sapiens, Rattus norvegicus
Toxicol. Appl. Pharmacol.
159
134-141
1999
-
-
-
-
-
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3
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6
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4
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10
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2
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2
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3
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4
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10
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2
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2
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646077
Bora
Mutagenesis and characterizati ...
Homo sapiens
Mol. Cell. Biochem.
180
111-115
1998
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1
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3
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1
1
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1
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1
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646078
Kaphalia
Purification and characterizat ...
Rattus norvegicus
Chem. Res. Toxicol.
10
211-218
1997
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1
1
1
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2
1
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2
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1
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7
1
1
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1
1
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1
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1
1
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1
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1
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7
1
1
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1
1
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1
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646071
Treloar
Fatty acid ethyl ester synthes ...
Homo sapiens
Biochim. Biophys. Acta
1299
160-166
1996
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2
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2
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1
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646073
Aleryani
Fatty acid ethyl ester synthas ...
Homo sapiens
Clin. Chem.
42
24-27
1996
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1
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2
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2
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1
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646079
Bora
Purification and characterizat ...
Homo sapiens
J. Mol. Cell. Cardiol.
28
2027-2032
1996
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2
3
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1
1
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1
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1
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646072
Heith
Fatty acid ethyl ester synthas ...
Homo sapiens
Biochem. Biophys. Res. Commun.
208
549-554
1995
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1
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646068
Tsujita
Fatty acid ethyl ester synthas ...
Rattus norvegicus
J. Biol. Chem.
267
23489-23494
1992
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1
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1
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2
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8
1
1
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1
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646067
Sharma
Independent segregation of glu ...
Homo sapiens
Biochem. J.
275
507-513
1991
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1
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646069
Bora
Molecular cloning, sequencing, ...
Homo sapiens
J. Biol. Chem.
266
16774-16777
1991
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1
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1
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1
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4
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1
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1
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646066
Riley
Pancreatic cholesterol esteras ...
Homo sapiens
Biochemistry
29
3848-3852
1990
1
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1
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2
1
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3
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1
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10
1
1
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1
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1
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10
1
1
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646065
Bora
Purification to homogeneity an ...
Homo sapiens
FEBS Lett.
258
236-239
1989
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5
2
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2
1
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2
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3
2
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7
1
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7
1
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646062
Mogelson
Nonoxidative ethanol metabolis ...
Oryctolagus cuniculus
Biochemistry
23
4075-4081
1984
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3
9
1
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2
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2
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12
1
1
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12
1
1
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646063
Mogelson
Partial purification and produ ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
798
144-148
1984
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2
2
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646064
Mogelson
Thermodynamic bases for fatty ...
Oryctolagus cuniculus
Biochemistry
23
4082-4087
1984
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1
1
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1
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